A Novel Type of Zinc Finger DNA Binding Domain in the Agrobacterium tumefaciens Transcriptional Regulator Ros (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• A Novel Type of Zinc Finger DNA Binding Domain in the Agrobacterium tumefaciens Transcriptional Regulator Ros (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1021/bi060697m (literal)

Alternative label

• S. Esposito, I. Baglivo, G. Malgieri, L. Russo, L. Zaccaro, LD. D'Andrea, M. Mammucari, B. Di Blasio, C. Isernia, R. Fattorusso and PV Pedone (2006)
  A Novel Type of Zinc Finger DNA Binding Domain in the Agrobacterium tumefaciens Transcriptional Regulator Ros
  in Biochemistry (Easton)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• S. Esposito, I. Baglivo, G. Malgieri, L. Russo, L. Zaccaro, LD. D'Andrea, M. Mammucari, B. Di Blasio, C. Isernia, R. Fattorusso and PV Pedone (literal)

Pagina inizio

• 10394. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 45 (literal)

Rivista

• Biochemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Pubblicazione su rivista scientifica (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• S. Esposito, Univ Naples 2, Dipartimento Sci Ambientali, I-81100 Caserta, Italy I. Baglivo, Univ Naples 2, Dipartimento Sci Ambientali, I-81100 Caserta, Italy G. Malgieri, Univ Naples 2, Dipartimento Sci Ambientali, I-81100 Caserta, Italy L. Russo, Univ Naples 2, Dipartimento Sci Ambientali, I-81100 Caserta, Italy L. Zaccaro, CNR, Ist Biostrutture & Bioimmagini, I-80134 Naples, Italy LD. D'Andrea, CNR, Ist Biostrutture & Bioimmagini, I-80134 Naples, Italy M. Mammucari, Univ Naples 2, Dipartimento Sci Ambientali, I-81100 Caserta, Italy B. Di Blasio, Univ Naples 2, Dipartimento Sci Ambientali, I-81100 Caserta, Italy C. Isernia, Univ Naples 2, Dipartimento Sci Ambientali, I-81100 Caserta, Italy R. Fattorusso,Univ Naples 2, Dipartimento Sci Ambientali, I-81100 Caserta, Italy PV Pedone, Univ Naples 2, Dipartimento Sci Ambientali, I-81100 Caserta, Italy (literal)

Titolo

• A Novel Type of Zinc Finger DNA Binding Domain in the Agrobacterium tumefaciens Transcriptional Regulator Ros (literal)

Abstract

• Transcriptional factors bearing a Cys(2)His(2) zinc finger were thought to be confined to eukaryotes, but recent studies have suggested their presence also in prokaryotes. In this paper, we report the first complete functional characterization of the DNA binding domain present in the putative Cys(2)His(2) zinc finger-containing prokaryotic transcriptional regulator Ros from Agrobacterium tumefaciens. We demonstrate that in the single zinc binding motif present in the Ros protein the metal ion is coordinated by two cysteines (Cys79 and Cys82) and two histidines (His92 and His97), separated by a shorter spacer with respect to the eukaryotic classical Cys(2)His(2) domains. The Cys(2)His(2) zinc finger motif is essential for Ros DNA binding and is part of a larger DNA binding domain which includes four basic regions located on either side of the finger, one at the N-terminus and three at the C-terminus. The one described here is a novel type of DNA binding domain containing a noncanonical Cys(2)His(2) zinc finger motif which, by sequence alignment, seems to be conserved in all the bacterial putative zinc finger proteins identified so far. Interestingly, basic amino acids have been shown to be important in stabilizing the DNA binding of eukaryotic single Cys(2)His(2) zinc finger domains, confirming that the modality of DNA binding using a single zinc finger motif flanked by basic residues is widespread throughout the living kingdom from eukaryotic, both animal and plant, to prokaryotic, even if in each kingdom it presents its peculiarity. (literal)

Prodotto di

• Determinazione della struttura tridimensionale di biomolecole complesse con tecniche spettroscopiche e cristallografiche (PM.P01.022.001) (Modulo)
• Institute of biostructure and bioimaging (IBB) (Istituto)
• Sistemi sintetici riconoscibili da target biologici (PM.P01.003.001) (Modulo)

Autore CNR

• LUCA DOMENICO D'ANDREA (Unità di personale interno)
• LAURA ZACCARO (Persona)

Insieme di parole chiave

• Keywords of "A Novel Type of Zinc Finger DNA Binding Domain in the Agrobacterium tumefaciens Transcriptional Regulator Ros" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of biostructure and bioimaging (IBB) (Istituto)
• Determinazione della struttura tridimensionale di biomolecole complesse con tecniche spettroscopiche e cristallografiche (PM.P01.022.001) (Modulo)
• Sistemi sintetici riconoscibili da target biologici (PM.P01.003.001) (Modulo)

Autore CNR di

• LUCA DOMENICO D'ANDREA (Unità di personale interno)
• LAURA ZACCARO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biochemistry (Rivista)

Insieme di parole chiave di

• Keywords of "A Novel Type of Zinc Finger DNA Binding Domain in the Agrobacterium tumefaciens Transcriptional Regulator Ros" (Insieme di parole chiave)