http://www.cnr.it/ontology/cnr/individuo/prodotto/ID135414

Improvements in fluorescence microscopy allowed by high power light emitting diodes. (Contributo in volume (capitolo o saggio))

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Improvements in fluorescence microscopy allowed by high power light emitting diodes. (Contributo in volume (capitolo o saggio)) (literal)

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Mazzini G., Ferrari C., Baraldo N., Mazzini M., Angelini M. (2004)
Improvements in fluorescence microscopy allowed by high power light emitting diodes.
A. Mendez-Vilas, L.Labajos-Broncano, Badajoz, Spain, 2004
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Fluorescence excitation, even in modern microscopes, is generally achieved by means of arc lamps. The peculiar characteristics of high energy and whole spectrum of emission of these lamps are associated with some disadvantages in terms of safety and global efficiency. High pressure Hg and Xe lamps in some circumstances may in fact explode. Furthermore among the total electrical power, less than 1% goes to the sample while the rest 99% is wasted or dispersed through heat. A new high efficient illumination system is proposed based on the use of high power light emitting diodes (LEDs). Both 1 and 5W Luxeon Star LEDs have been employed. A specific plastic light condenser lens have been designed and made by Fraen. Blue and green LEDs have been tested to generate the corresponding two excitation bands. These light beams have been compared with similar bands delivered by the HB0 100 W lamp. The measurements of light intensities at the objective lens output allow to state that the Luxeon STAR LEDs can deliver a light beam of radiant intensity similar to the blue and green bands delivered by the standard HBO 100W lamp. Based on these experiments a prototype of an advanced, all solid state, illuminator had been realized. It can replace conventional excitation arc lamp system as well as up-grade conventional bright field instruments to fluorescence microscopes. (literal)

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Histochemistry and Cytometry Sect. IGM-CNR, Dept.of Animal Biology University of PAVIA-Italy.; Dept. of Surgery Epatopancreatic Section University of Pavia-Italy, Fraen Corporation Milan-Italy. (literal)

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