http://www.cnr.it/ontology/cnr/individuo/prodotto/ID132458
Advances in marine bacterial pollution monitoring (Contributo in volume (capitolo o saggio))
- Type
- Label
- Advances in marine bacterial pollution monitoring (Contributo in volume (capitolo o saggio)) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Alternative label
Caruso G.; Crisafi E.; Caruso R.; Zappalà G. (2008)
Advances in marine bacterial pollution monitoring
Nova Science Publishers, Hauppauge (Stati Uniti d'America) Nova Science Publishers, New York (Stati Uniti d'America) in Environmental Microbiology Resaerch Trends, 2008
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Caruso G.; Crisafi E.; Caruso R.; Zappalà G. (literal)
- Pagina inizio
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#citta
- Hauppauge, NY. USA (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume
- Environmental Microbiology Resaerch Trends (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- G. V. Kurladze (Editor), Chapter 10, pp. 273-287. (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- CNR - Istituto per l'ambiente marino costiero (literal)
- Titolo
- Advances in marine bacterial pollution monitoring (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#inCollana
- Environmental Microbiology Research Trends (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn
- 978-1-60021-939-9 (literal)
- Abstract
- Coastal aqualic environments often suffer from anthropogenic pressure, posing
severe threals lo human health related to presence of pollutants in seawaters. Recent
trends in environmental monitoring aiming at public health preservation highlight the
need of a deep revision of the standards up to now used in the assessment of seawater
quality, focusing the importance of new bacterial indicators, more reliable than those
previously used, in the contro I of short and large scale-occurring pollution phenomena.
Selected bacteri al species (Escherichia coli) or related parameters are currently included
in monitoring programs of marine environmelltal quality; they allow to track the
occurrence and evolution of bacterial pollution events, and prevent human health risks
related to the use of polluted waters. Moreover, several limitations intrinsic to culture
methods traditionally used to detect bacterial pathogens (long response times, low
specificity, high costs) confirrn their inadequacy for coastal monitoring, stressing the
need for updated standard analytical procedures. In response to this, several research
efforts at the CNR - IAMC have recelltly been devoted to improve technical equipmellts
and methodologies for the assessment of microbiological quality of seawater. The cbapter
reviews recent advances in coastal microbial pollution rnonitoring achieved in the
framework of projects funded by the Italian Ministry for Scientific Rescarch, addrcssing
the detection and enumeration of Escherichia coli in seawater as an indicator of faecal
pollution. A summary of the innovative methodologies (the fluorescent antibody method
the P-glucuronidase assay and Real time PCR) developed and optimised at the LAMe
laboratory for monitoring bathing waters is reported, which allowed thc high-resolution
quantitative measurement of target bacterial molecules and accurate quantification of
faecal pollution phenomena. These methods provided results significantly related to plate
counts within a few hours from sampling, highlighting the main advantages (rapidity and
specificity) related to the detection of bacterial antigens or molecules as markers of faecal
pollulion. New combined fluorescence-viability staining protocols were also set up,
capable to detect bacteria which persist into seawater under different physiological states,
maintain ing intact living properties, and therefore have a pathogenic potential. The
proposed methods proved to be rapid and specific analytic approaches for the screening
of coastal areas with various degrees of pollution; therefore they offer new interesting
perspectives to prevenl outbreaks of waterbome diseases. Data obtained by these new
analytical procedures encourage the use of E. coli or related parameters as successful
tools for early waming of seawater bacterial pollution. (literal)
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