http://www.cnr.it/ontology/cnr/individuo/prodotto/ID130939
Studies on the replication of Tombusvirus Satellite RNAs (Abstract/Poster in convegno)
- Type
- Label
- Studies on the replication of Tombusvirus Satellite RNAs (Abstract/Poster in convegno) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- RUBINO L. & RUSSO M. (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- 7-11 aprile 2010 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
- Background. Plant virus infections are often associated with subviral RNA molecules, constituted by defective interfering (DI) or satellite (sat) RNAs. Both these RNAs are totally dependent on helper virus-encoded factors for their replication but are dispensable for virus propagation. DI RNAs are shortened forms of the viral genome, generated by discontinuities of the viral replicase and deprived of all viral genes required for replication. SatRNAs share little or no sequence homology with the helper virus genome. Three satRNAs associated with members of the genus Tombusvirus have been described so far. They are linear, single-stranded non-coding RNAs c. 600-800 nt in size, with sequence homology with the helper virus limited to a 50 nt stretch in the 5 non coding region of all tombusviral genomes and DI RNAs.
Methods: The replication of different satRNAs was tested in plant and protoplasts, and the presence of satRNA-derived siRNA was analyzed.
Results: The biological properties of satRNA L, a novel satRNA associated with TBSV were described. Tombusvirus satRNAs differed in the ability of being replicated by the viral replicase expressed in transgenic Nicotiana benthamiana plants. The relevance of the genomic ORF1 product for satRNA L replication was investigated using different hybrid tombusviruses as helper. The accumulation of siRNAs derived from satRNAs and from genomic RNA depended on the temperature and on the length (time) of infection.
Conclusions: SatRNA L and B10, a different satRNA associated with natural Tomato bushy stunt virus (TBSV) infections, are both initiators and targets of RNA silencing.
(literal)
- Titolo
- Studies on the replication of Tombusvirus Satellite RNAs (literal)
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