Dissection of the viroid molecular determinant inducing peach calico disease. (Abstract/Poster in atti di convegno)

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  • Dissection of the viroid molecular determinant inducing peach calico disease. (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Navarro B., Delgado S., Rodio M.E., Flores R. and F. Di Serio (2009)
    Dissection of the viroid molecular determinant inducing peach calico disease.
    in I Congresso della Società Italiana di Biologia Vegetale, Verona
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Navarro B., Delgado S., Rodio M.E., Flores R. and F. Di Serio (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Viroids, infectious non-protein-coding RNAs, may cause plant diseases by a mechanism still unknown. Peach latent mosaic viroid (PLMVd), which replicates and accumulates in the chloroplast, has been proposed as a model for studying viroid pathogenesis. We recently showed that peach calico (PC), a severe chlorosis exclusively elicited by PLMVd variants containing a specific insertion of 12-13 nt, is due to the impairment of plastid ribosomal RNA maturation at a very early developmental stage. Here, we have further dissected the 12-13 nt insertion by site-directed mutagenesis, bioassay of the resulting variants and molecular analysis of the progenies. Our new data reveal that not only the size but also the nucleotide composition and conformation of the insertion strongly affect the pathogenic properties of PLMVd infecting variants. This information can be relevant for identifying host factor(s) involved in symptom elicitation interacting directly with the pathogenic determinant, and for exploring the involvement of RNA silencing in viroid pathogenesis, as recently suggested in other viroid-host combinations. In the present study, we have also observed that new PLMVd variants, most likely non-pathogenic, emerge from the inoculated PC-inducing variants, further supporting the use of PLMVd as a model system for studying pathogenesis and evolution of non-protein-coding RNAs. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC),Valencia, Spain. (literal)
Titolo
  • Dissection of the viroid molecular determinant inducing peach calico disease. (literal)
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