New aspects of the interaction between thrips and tospovirus (Comunicazione a convegno)

Type
Label
  • New aspects of the interaction between thrips and tospovirus (Comunicazione a convegno) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Ciuffo, M., Margaria,P., Mautino,G., Bosco, D., Tavella , L., Turina, M. (2009)
    New aspects of the interaction between thrips and tospovirus
    in XV Convegno nazionale della Società Italiana di Patologia Vegetale, Locorotondo (Bari)
    (literal)
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  • Ciuffo, M., Margaria,P., Mautino,G., Bosco, D., Tavella , L., Turina, M. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Western flowers thrips (Frankliniella occidentalis) is the main vector of Tomato spotted wilt virus (TSWV), the type member of the genus Tospovirus. The virus is transmitted in a persistent propagative manner. In order to characterize NSs-defective strains, we forced the obtainment from a wild-type strain (p202/3WT) of a NSs truncated strain by mechanical inoculation on resistant pepper. The strain we obtained was named p202/3RB and carried a single nucleotide mutation (G deletion) in the NSs coding region, resulting in a frame-shift and a truncated NSs protein of 443aa (compared to 467aa of p202/3WT). Transmission experiment in leaf disk assay using the two strain (WT and RB) showed a percentage of transmission higher then 50% in P202/3 WT, whereas 0% in RB. Quantitative RT-PCR assays on the adult thrips immediately after the third inoculation access period (IAP) allowed us to detect a very low titer of viral RNA for p202/3RB compared to a much more abundant p202/3WT viral RNA. Sequence comparison of the genomic segments of the two related strains showed 100% homology for the S segment with the exception of the G deletion and 100% homology for the M segment. Taken together our data provide genetic evidence for the involvement of the S segment in the TSWV-thrips relationship and for a role of NSs in efficient vector infection. In a different experiment we evaluated the number of thrips able to transmit wild type TSWV after each of four subsequent IAPs. Among 200 adults analyzed about 70% could transmit at the first passage and surprisingly 5% of individuals stopped to transmit after the first passage indicating a possible individual recovery from viral infection. (literal)
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  • Di.Va.P.R.A. Entomologia e Zoologia applicate all’Ambiente, University of Torino, Via L. da Vinci 44, 10095 Grugliasco (TO), Italy (literal)
Titolo
  • New aspects of the interaction between thrips and tospovirus (literal)
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