http://www.cnr.it/ontology/cnr/individuo/prodotto/ID130870

Deep sequencing of Vitis vinifera short RNAs reveals non-conserved mi RNAs and a number infectious agents-deriving siRNAs. (Abstract/Poster in atti di convegno)

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Deep sequencing of Vitis vinifera short RNAs reveals non-conserved mi RNAs and a number infectious agents-deriving siRNAs. (Abstract/Poster in atti di convegno) (literal)

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V. Pantaleo(1), G. Szittya (2), L. Miozzi (1), S. Moxon(2), T. Dalmay (2), J. Burgyan (1). (2009)
Deep sequencing of Vitis vinifera short RNAs reveals non-conserved mi RNAs and a number infectious agents-deriving siRNAs.
in 1° Congresso Società Italiana di Biologia Vegetale, Verona
(literal)

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V. Pantaleo(1), G. Szittya (2), L. Miozzi (1), S. Moxon(2), T. Dalmay (2), J. Burgyan (1). (literal)

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Grapevine (Vitis vinifera) is one of the most ancient and important fruit crops. V. vinifera is an host of a large number of viral and virus-like agents including phytoplasmas, viroids and viruses either associated with diseases or at a latency state. In late 2007 a major achievement has been reached in V.vinifera biology since the sequence of the grapevine genome has been published and it is available for public research. Thus it prompted us to use the grapevine as a natural system to investigate some molecular aspects of plant-virus interactions involved in the plant development. In this work we present the results of deep sequencing analysis of short RNAs in grapevine clone ENTAV15. Infectious agent-deriving siRNAs either from viruses or viroids were cloned and located along their originating genome sequences, thus revealing siRNAs site- and strand-dependent distribution. Moreover, 21 novel miRNAs and their star molecules were cloned; bioinformatics analysis either of their precursors and predicted targets, and northern blot analysis for their expression in different tissues likely suggest the identification of novel non-conserved miRNAs. (literal)

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(1) Istituto di Virologia Vegetale del CNR, Strada delle Cacce 73 10135 Torino (Italy) (2) School of Biological Sciences, University of East Anglia, Norwich, (United Kingdom) (literal)

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