An RGG motif in the Rep protein of Tomato yellow leaf curl Sardinia virus is important for transcriptional repression, nuclear localisation and resistance. (Abstract/Poster in atti di convegno)

Type

• Abstract/Poster in atti di convegno (Classe)
• Prodotto della ricerca (Classe)

Label

• An RGG motif in the Rep protein of Tomato yellow leaf curl Sardinia virus is important for transcriptional repression, nuclear localisation and resistance. (Abstract/Poster in atti di convegno) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• Sardo, L., Masenga, V., Lucioli, A., Tavazza, M., Fammartino, A., Tavazza, R., Vaira, A.M, Accotto, G.P., Noris, E. (2008)
  An RGG motif in the Rep protein of Tomato yellow leaf curl Sardinia virus is important for transcriptional repression, nuclear localisation and resistance.
  in EMBO Workshop 2008, The Nucleous and Disease, Derby, Great Britain, June, 23-25, 2008
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Sardo, L., Masenga, V., Lucioli, A., Tavazza, M., Fammartino, A., Tavazza, R., Vaira, A.M, Accotto, G.P., Noris, E. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Geminiviruses, seriously threatening several crops worldwide, have a circular single-stranded DNA genome replicating in the nucleus with the aid of the replication associated protein (Rep). C-terminally truncated Reps of 210 or 130 amino acids (Rep-210 and -130) of Tomato yellow leaf curl Sardinia virus (TYLCSV) confer resistance to virus infection by inhibiting virus transcription and replication.These functions are not inhibited if a further terminal 10 aa are deleted (Rep-120; Lucioli et al., 2003). By analysing more than 560 Begomovirus Reps, a conserved RGG motif (aa 124-126) was detected in this 10-aa stretch. RGG motifs are involved in RNA binding, transcriptional repression, protein-protein interaction and nuclear/nucleolar localization. Rep-210 and -130 RGG to SAA mutants were produced. They showed reduced transcriptional inhibitory activity in transfected protoplasts compared to their non-mutated counterparts. Instead, only Rep-130 SAA, but not Rep-210 SAA, had a reduced ability to inhibit virus replication. When overexpressed in transgenic plants, Rep-210 and -130 were immunolocalised in the nucleus and nucleolus, while Rep-120, -130 SAA and -210 SAA barely accumulated above background. Furthermore, transgenic plants expressing Rep-210 SAA and -130 SAA showed reduced resistance to TYLCSV. Thus, the RGG mutation affects different activities of TYLCSV Rep. (literal)

Note

• Poster (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• LA, TM, TR: CRE Casaccia, ENEA,Via Anguillarese 301, 00123 Santa Maria di Galeria, Roma, Italy SL, MV, FA, VAM, AGP, NE: IVV CNR, Str delle Cacce 73, 10135 Turin Italy (literal)

Titolo

• An RGG motif in the Rep protein of Tomato yellow leaf curl Sardinia virus is important for transcriptional repression, nuclear localisation and resistance. (literal)

Abstract

• Geminiviruses, seriously threatening several crops worldwide, have a circular single-stranded DNA genome replicating in the nucleus with the aid of the replication associated protein (Rep). C?terminally truncated Reps of 210 or 130 amino acids (Rep-210 and -130) of Tomato yellow leaf curl Sardinia virus (TYLCSV) confer resistance to virus infection by inhibiting virus transcription and replication.These functions are not inhibited if a further terminal 10 aa are deleted (Rep-120; Lucioli et al., 2003). By analysing more than 560 Begomovirus Reps, a conserved RGG motif (aa 124-126) was detected in this 10-aa stretch. RGG motifs are involved in RNA binding, transcriptional repression, protein-protein interaction and nuclear/nucleolar localization. Rep-210 and -130 RGG to SAA mutants were produced. They showed reduced transcriptional inhibitory activity in transfected protoplasts compared to their non-mutated counterparts. Instead, only Rep-130 SAA, but not Rep-210 SAA, had a reduced ability to inhibit virus replication. When overexpressed in transgenic plants, Rep-210 and -130 were immunolocalised in the nucleus and nucleolus, while Rep-120, -130 SAA and -210 SAA barely accumulated above background. Furthermore, transgenic plants expressing Rep-210 SAA and -130 SAA showed reduced resistance to TYLCSV. Thus, the RGG mutation affects different activities of TYLCSV Rep. (literal)

Prodotto di

• Resistenze convenzionali e non-convenzionali a importanti virosi e fitoplasmosi di specie coltivate (AG.P01.014.001) (Modulo)

Autore CNR

• EMANUELA NORIS (Unità di personale interno)
• GIAN PAOLO ACCOTTO (Unità di personale interno)
• VERA MASENGA (Persona)
• Luca SARDO (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "An RGG motif in the Rep protein of Tomato yellow leaf curl Sardinia virus is important for transcriptional repression, nuclear localisation and resistance." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• GIAN PAOLO ACCOTTO (Unità di personale interno)
• VERA MASENGA (Persona)
• EMANUELA NORIS (Unità di personale interno)
• Luca SARDO (Unità di personale esterno)

Prodotto

• Resistenze convenzionali e non-convenzionali a importanti virosi e fitoplasmosi di specie coltivate (AG.P01.014.001) (Modulo)

Insieme di parole chiave di

• Keywords of "An RGG motif in the Rep protein of Tomato yellow leaf curl Sardinia virus is important for transcriptional repression, nuclear localisation and resistance." (Insieme di parole chiave)