Cymbidium Ringspot Virus Controls The Accumulation Of Subviral SatRNA By Virus Induced Gene Silencing (Comunicazione a convegno)

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  • Cymbidium Ringspot Virus Controls The Accumulation Of Subviral SatRNA By Virus Induced Gene Silencing (Comunicazione a convegno) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Alternative label
  • Pantaleo, V., (1) Burgyan, J. (2007)
    Cymbidium Ringspot Virus Controls The Accumulation Of Subviral SatRNA By Virus Induced Gene Silencing
    in EMBO Central European Scientists Meeting, Tallin, Estonia
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Pantaleo, V., (1) Burgyan, J. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Satellite RNA (Cym satRNA) of Cymbidium ringspot virus (CymRSV) is a sub-viral RNA replicon found associated with its helper virus. It consists of a 621nt long RNA species sharing only a 50 nt long high similarity level sequence (termed Helper-Satellite Homology region-HSH) with the helper virus. Here we show that its accumulation depends on the temperature and the presence of p19 silencing suppressor protein of helper virus, suggesting that RNA silencing plays a crucial role in the accumulation of satRNA. We have demonstrated that Carnation Italian ringspot virus (CIRV), another Tombusvirus member, can support the Cym satRNA accumualtion at higher level than CymRSV. Our results suggest that siRNA derived from CIRV can target satRNA less efficiently than siRNAs from CymRSV. This finding was further supported by mutational analysis of the predicted helper viral siRNA-originating site, which is supposed to drive the cleavage of the satRNA in the HSH. Indeed we have shown that the two mismatches occurring in the HSH sequence are responsible for the control of Cym satRNA accumulation level. All together these results, led us to conclude that CymRSV can induce RNA silencing by producing siRNA specifically raised to down regulate the Cym satRNA accumulation. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • (1) Agricultural Biotechnology Center, Godollo (Hungary) (literal)
Titolo
  • Cymbidium Ringspot Virus Controls The Accumulation Of Subviral SatRNA By Virus Induced Gene Silencing (literal)
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