Development of in vitro Propagation, Slow Growth Storage and Cryopreservation Protocols for Short-, Medium- and Long-Term Conservation of Young and Mature Explants of Thyme (Thymus vulgaris and T. longicaulis) (Abstract/Comunicazione in atti di convegno)

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  • Development of in vitro Propagation, Slow Growth Storage and Cryopreservation Protocols for Short-, Medium- and Long-Term Conservation of Young and Mature Explants of Thyme (Thymus vulgaris and T. longicaulis) (Abstract/Comunicazione in atti di convegno) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Alternative label
  • Ozudogru E.A., Kaya E., Kirdok E. (2010)
    Development of in vitro Propagation, Slow Growth Storage and Cryopreservation Protocols for Short-, Medium- and Long-Term Conservation of Young and Mature Explants of Thyme (Thymus vulgaris and T. longicaulis)
    in IHC - 28th International Horticultural Congress, Lisbon, Portugal
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Ozudogru E.A., Kaya E., Kirdok E. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • 21-27 August 2010, Abstracts: Vol. II, p. 525. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Thyme is one of the most important genus of the Lamiaceae family, covering more than 400 perennial species of aromatic and medicinal shrubs/subshrubs. It serves as a natural source of phenolic oils, oleoresins, fresh and dried herbs. Non-medicinal use of the plant (e.g., food additives, cosmetic and alimentary industries, landscape plants) is also of great importance. However, natural thyme populations are unfortunately far from being adequate to support such a great and even growing demand for its products. In this sense, recently developed in vitro propagation techniques provide a unique alternative for the high-rate multiplication of plants of horticultural, economic and medicinal importance, and pave the way to medium- and long-term conservation of such valuable germplasm by means of slow growth storage and cryopreservation. Present study was designated to develop effective protocols for in vitro propagation, slow growth storage and cryopreservation of young (i.e., shoot tips originating from young seedlings) and mature explants (i.e., shoot tips originated from mature plants) of two distant Thymus species, i.e., T. vulgaris and T. longicaulis, in order to ensure its applicability to a wide range of thyme germplasm. Optimizations for in vitro shoot proliferation included use of cytokinins (6-benzyladenine, kinetin and thidiazuron) alone or in combination with auxins, gibberellic acid and/or silver nitrate. Shoot rooting was easily obtained on semi-solid MS medium, either hormone-free or supplemented with auxins. Rooted plantlets were transferred to 250-ml plastic pots and acclimatized successfully by gradually reducing the relative humidity. Slow growth storage was achieved by keeping the in vitro shoot cultures at 4 °C and in darkness up to 12 months. Three different vitrification-based one-step freezing techniques (PVS2 vitrification, encapsulation-vitrification and droplet-freezing) were tested for cryopreservation, however initially all the preliminary steps of cryoprotection (i.e.,cold- hardening, sucrose preculture) were also optimized. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Ozudogru E.A., Kaya E., Kirdok E.: Gebze Institute of Technology, Istanbul Caddesi, No: 101, 41400, Gebze-Kocaeli, Turkey (literal)
Titolo
  • Development of in vitro Propagation, Slow Growth Storage and Cryopreservation Protocols for Short-, Medium- and Long-Term Conservation of Young and Mature Explants of Thyme (Thymus vulgaris and T. longicaulis) (literal)
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