http://www.cnr.it/ontology/cnr/individuo/prodotto/ID130443

Development of cryopreservation procedures for dwarf irises (Iris spp.) (Comunicazione a convegno)

Type

Prodotto della ricerca (Classe)
Comunicazione a convegno (Classe)

Label

Development of cryopreservation procedures for dwarf irises (Iris spp.) (Comunicazione a convegno) (literal)

Anno

2009-01-01T00:00:00+01:00 (literal)

Alternative label

Jevremovic S., Benelli C., De Carlo A., Subotic A., Lambardi M. (2009)
Development of cryopreservation procedures for dwarf irises (Iris spp.)
in 1st International Symposium on Cryopreservation in Horticultural Species, Leuven, Belgio
(literal)

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Jevremovic S., Benelli C., De Carlo A., Subotic A., Lambardi M. (literal)

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Crioconservazione di germogli di iris. (literal)

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Aiming to optimize cryopreservation procedures for shoot tips and embryogenic calli of two dwarf iris species (I. pumila and I. reichenbachii), several experiments were carried out using the vitrification, the encapsulation-dehydration and the droplet- vitrification methods. Best results were obtained with the vitrification method for shoot tips and the encapsulation-dehydration method for embryogenic calli. Shoot tips, excised from in vitro shoot cultures of irises, were successfully cryopreserved after the development of a vitrification procedure based on the treatment with the PVS2 solution. Following thawing and plating, survived shoot tips resumed growth within 3 weeks without any production of callus, and afterwards developed in well-formed shoots within 5 weeks. Maximum shoot survivals (75% for I. pumila and 93% for I. reichenbachii) were achieved when the shoot tips were treated with PVS2 for 15-20 min at 25°C before the direct immersion in liquid nitrogen. However, in terms of shoot development and further rapid shoot multiplication, best results (55%) were achieved for I. pumila when shoot tips are treated with PVS2 for 20 min at 0°C. When the encapsulation-dehydration method was applied, best survival (63%) was achieved with I. reichenbachii shoot tips. Experiments are still in progress to develop also a droplet-vitrification method for shoot-tip cryopreservation, with promising preliminary results. For cryopreservation of embryogenic calli, the possibility to dessicate samples of non-encapsulated and encapsulated calli in 3% alginate beads was first evaluated. Non-encapsulated and encapsulated callus samples of both the species showed to tolerate well the exposure to the sterile air of a laminar flow hood for up to 4 hours. When longer dessication times were applied, proliferation activity was resumed only by I. reichenbachii calli. Among the different iris callus typologies, a yellow-white type showed the highest morphogenetic potential after dehydration and regrowth on 2,4-D containing medium. Experiments on conservation in liquid nitrogen of callus samples, following the application of the developed dehydration procedure, are in progress. (literal)

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