In vivo and in vitro functional study of Tbx3, the gene mutated in the Ulnar Mammary sindrome (Abstract/Poster in atti di convegno)

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Label
  • In vivo and in vitro functional study of Tbx3, the gene mutated in the Ulnar Mammary sindrome (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Alternative label
  • Bertoli G., Scotti M., Sanzone S., Valsecchi V., Pelucchi P., Platonova N., Merlo G.R., and Zucchi I. (2007)
    In vivo and in vitro functional study of Tbx3, the gene mutated in the Ulnar Mammary sindrome
    in Telethon Convention, Salsomaggiore Terme
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Bertoli G., Scotti M., Sanzone S., Valsecchi V., Pelucchi P., Platonova N., Merlo G.R., and Zucchi I. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • TBX3 encodes for a transcription factor of the T-box gene family, defined by the DNA binding sequence T-box. Mutations, which cause haploinsufficiency of TBX3, generate the Ulnar-Mammary Syndrome (UMS, OMIM 181450), an autosomal dominant disorder characterized by upper limb skeletal malformations and aplasia or hypoplasia of breast, teeth, hair and apocrine glands. To define the role of TBX3 in cell growth and differentiation, we modulated TBX3 expression levels in mammary epithelial cells (MECs) by Antisense oligonucleotide (As) treatment. TBX3 depletion in LA7 cells (rat MECs that recapitulate alveolo- and tubulo-genesis in vitro) leads to mammary cells differentiation, and alters cell proliferation, suggesting a role of TBX3 in growth regulation. TBX3 As treatment inhibits strongly both anchorage-independent colony formation and growth of the human MECs, MDA435 and BT474, while their differentiation and apoptosis are not affected. This growth regulation appears not to be dependent on the p53 status (previously shown with wt or mutated p53). To detect TBX3 co-regulated genes, total RNA of TBX3-depleted cells have been used for micro-array analysis. In parallel, since T-box target -gene-specificity could be to be controlled by cell-type specific co-factors, in collaboration with H. Shöler’s group, we are identifying Tbx3 co-factors involved in cells growth control, by using chromatin immunoprecipitation (ChIP), two dimensional protein electrophoresis and proteomic analyses. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institute of Biomedical Technologies, National Research Council, Via Cervi 93, 20090 Segrate-Milan, Italy. (literal)
Titolo
  • In vivo and in vitro functional study of Tbx3, the gene mutated in the Ulnar Mammary sindrome (literal)
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