Pyruvate kinase in pig liver mitochondria (Articolo in rivista)

Type
Label
  • Pyruvate kinase in pig liver mitochondria (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.abb.2009.12.016 (literal)
Alternative label
  • Pizzuto R, Paventi G, Atlante A, Passarella S. (2010)
    Pyruvate kinase in pig liver mitochondria
    in Archives of biochemistry and biophysics (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Pizzuto R, Paventi G, Atlante A, Passarella S. (literal)
Pagina inizio
  • 42 (literal)
Pagina fine
  • 48 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Impact factor ARCH BIOCHEM BIOPHYS ANNO 2010 : 3.022 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 495 (literal)
Rivista
Note
  • Scopu (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Dipartimento di Scienze per la Salute, Università del Molise, via De Sanctis - 86100 Campobasso, Italy Dipartimento di Scienze Animali, Vegetali e dell'Ambiente, Università del Molise, via De Sanctis - 86100 Campobasso, Italy Istituto di Biomembrane e Bioenergetica, CNR, via G. Amendola 165/A - 70126 Bari, Italy (literal)
Titolo
  • Pyruvate kinase in pig liver mitochondria (literal)
Abstract
  • The existence of the pyruvate kinase (PK) in pig liver mitochondria was shown by monitoring photometrically the PK reaction in solubilised mitochondria with either phosphoenolpyruvate (PEP) or ADP used as a substrate. In distinction with the cytosolic isoenzyme, the mitochondrial PK showed a sigmoidal dependence on either PEP or ADP concentrations. The occurrence of the mitochondrial PK was confirmed by immunological analysis. Titration with digitonin showed that mPK is restricted to the matrix. PEP addition to mitochondria resulted in reduction of the intramitochondrial NAD(P)+ inhibited by either the nonpenetrant thiol reagent mersalyl or by arsenite, an inhibitor of the pyruvate dehydrogenase complex. Citrate/ oxaloacetate appearance outside mitochondria also occurred as result of PEP addition to PLM. Taken together these findings support a role for PEP itself in triggering fatty acid synthesis via its mitochondrial metabolism. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Prodotto
Autore CNR di
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it