Epigenetic mechanisms and Sp1 regulate mitochondrial citrate carrier gene expression. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Epigenetic mechanisms and Sp1 regulate mitochondrial citrate carrier gene expression. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• V. Iacobazzi, V. Infantino and F. Palmieri (2008)
  Epigenetic mechanisms and Sp1 regulate mitochondrial citrate carrier gene expression.
  in Biochemical and biophysical research communications (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• V. Iacobazzi, V. Infantino and F. Palmieri (literal)

Pagina inizio

• 15 (literal)

Pagina fine

• 20 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 376 (literal)

Rivista

• Biochemical and biophysical research communications (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Iacobazzi V Department of Pharmaco-Biology, University of Bari CNR, Institute of Bioenergetics and Biomembranes, Bari Infantio V Department of Pharmaco-Biology, University of Bari Palmieri F Department of Pharmaco-Biology, University of Bari CNR, Institute of Bioenergetics and Biomembranes, Bari (literal)

Titolo

• Epigenetic mechanisms and Sp1 regulate mitochondrial citrate carrier gene expression. (literal)

Abstract

• This study investigates the transcriptional role of the mitochondrial CIC proximal promoter. The wild-type (but not methylated) -335/-20 bp region of the CIC gene confers gene reporter activity, and the five wild-type (but not methylated) Sp1 binding elements in this region bind human recombinant Sp1. The DNA demethylating agent AzaC or the histone acetylating agent TSA increases CIC transcript and protein levels as well as the binding of Sp1 and of acetylated histones to the -335/-20 bp region of the CIC promoter in SK-N-SH cells but not in HepG2 cells; when untreated these cells exhibit low and high levels of gene expression, respectively. Finally, Sp1 silencing decreases proximal promoter-driven gene reporter activity as well as CIC mRNA and CIC protein in both untreated HepG2 cells and AzaC- and TSA-treated SK-N-SH cells. These results show that methylation, histone acetylation and Sp1 are important in the transcriptional regulation of the CIC proximal promoter (literal)

Prodotto di

• Trasportatori mitocondriali: struttura e meccanismi funzionali (SV.P14.007.001) (Modulo)
• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)

Autore CNR

• FERDINANDO PALMIERI (Unità di personale esterno)
• vito iacobazzi (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "Epigenetic mechanisms and Sp1 regulate mitochondrial citrate carrier gene expression." (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)
• Trasportatori mitocondriali: struttura e meccanismi funzionali (SV.P14.007.001) (Modulo)

Autore CNR di

• FERDINANDO PALMIERI (Unità di personale esterno)
• vito iacobazzi (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biochemical and biophysical research communications (Rivista)

Insieme di parole chiave di

• Keywords of "Epigenetic mechanisms and Sp1 regulate mitochondrial citrate carrier gene expression." (Insieme di parole chiave)