The mitochondrial citrate/isocitrate carrier plays a regulatory role in glucose-stimulated insulin secretion. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• The mitochondrial citrate/isocitrate carrier plays a regulatory role in glucose-stimulated insulin secretion. (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• J. W. Joseph, M. V. Jensen, O. Ilkayeva, F. Palmieri, C. Alárcon, C. J. Rhodes and C. B. Newgard (2006)
  The mitochondrial citrate/isocitrate carrier plays a regulatory role in glucose-stimulated insulin secretion.
  
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• J. W. Joseph, M. V. Jensen, O. Ilkayeva, F. Palmieri, C. Alárcon, C. J. Rhodes and C. B. Newgard (literal)

Pagina inizio

• 35624 (literal)

Pagina fine

• 32 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 281 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Laboratory of Biochemistry and Molecular Biology, Department of Pharmaco-Biology, University of Bari, Via Orabona 4, 70125 Bari, Italy (literal)

Titolo

• The mitochondrial citrate/isocitrate carrier plays a regulatory role in glucose-stimulated insulin secretion. (literal)

Abstract

• Glucose-stimulated insulin secretion (GSIS) is mediated in part by glucose metabolism-driven increases in ATP/ADP ratio, but by-products of mitochondrial glucose metabolism also play an important role. Here we investigate the role of the mitochondrial citrate/isocitrate carrier (CIC) in regulation of GSIS. Inhibition of CIC activity in INS-1-derived 832/13 cells or primary rat islets by the substrate analogue 1,2,3-benzenetricarboxylate (BTC) resulted in potent inhibition of GSIS, involving both first and second phase secretion. A recombinant adenovirus containing a CIC-specific siRNA (Ad-siCIC) dose-dependently reduced CIC expression in 832/13 cells and caused parallel inhibitory effects on citrate accumulation in the cytosol. Ad-siCIC treatment did not affect glucose utilization, glucose oxidation, or ATP/ADP ratio but did inhibit glucose incorporation into fatty acids and glucose-induced increases in NADPH/NADP(+) ratio relative to cells treated with a control siRNA virus (Ad-siControl). Ad-siCIC also inhibited GSIS in 832/13 cells, whereas overexpression of CIC enhanced GSIS and raised cytosolic citrate levels. In normal rat islets, Ad-siCIC treatment also suppressed CIC mRNA levels and inhibited GSIS. We conclude that export of citrate and/or isocitrate from the mitochondria to the cytosol is an important step in control of GSIS. (literal)

Prodotto di

• Institute of biomembrane and bioenergetics (IBBE) (Istituto)
• Trasportatori mitocondriali: struttura e meccanismi funzionali (SV.P14.007.001) (Modulo)

Autore CNR

• FERDINANDO PALMIERI (Unità di personale esterno)

Insieme di parole chiave

• Parole chiave di "The mitochondrial citrate/isocitrate carrier plays a regulatory role in glucose-stimulated insulin secretion." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of biomembrane and bioenergetics (IBBE) (Istituto)
• Trasportatori mitocondriali: struttura e meccanismi funzionali (SV.P14.007.001) (Modulo)

Autore CNR di

• FERDINANDO PALMIERI (Unità di personale esterno)

Insieme di parole chiave di

• Parole chiave di "The mitochondrial citrate/isocitrate carrier plays a regulatory role in glucose-stimulated insulin secretion." (Insieme di parole chiave)