http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12792
The Drosophila termination factor DmTTF regulates in vivo mitochondrial transcription (Articolo in rivista)
- Type
- Label
- The Drosophila termination factor DmTTF regulates in vivo mitochondrial transcription (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
- Alternative label
Roberti M., Bruni F., Loguercio Polosa P. , Gadaleta MN. , Cantatore P. (2006)
The Drosophila termination factor DmTTF regulates in vivo mitochondrial transcription
in Nucleic acids research
(literal)
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- Roberti M., Bruni F., Loguercio Polosa P. , Gadaleta MN. , Cantatore P. (literal)
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- pubblicazione scientifica di interesse internazionale (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Dipartimento di Biochimica e Biologia Molecolare Ernesto Quagliariello, Universita` di Bari. Istituto di
Biomembrane e Bioenergetica, CNR, Bari, Via Orabona 4, 70125, Bari (literal)
- Titolo
- The Drosophila termination factor DmTTF regulates in vivo mitochondrial transcription (literal)
- Abstract
- DmTTF is a Drosophila mitochondrial DNA-binding protein, which recognizes two sequences placed at the boundary of clusters of genes transcribed in
opposite directions. To obtain in vivo evidences on the role of DmTTF, we characterized a DmTTF knockdown phenotype obtained by means of RNA interference in D.Mel-2 cells. By a combination of RNase protection
and real-time RTPCR experiments we found that knock-down determines remarkable changes in mitochondrial transcription. In particular, protein
depletion increases not only the level of (+) and (-)strand RNAs mapping immediately after of the two protein-binding site, but also that of transcripts located further downstream. Unexpectedly, depletion
of the protein also causes the decrease in the content of those transcripts mapping upstream of the protein target sites, including the two rRNAs. The changes in transcript level do not depend on a variation in
mitochondrial DNA (mtDNA) content, since mtDNA copy number is unaffected by DmTTF depletion. This work shows conclusively that DmTTF arrests
in vivo the progression of the mitochondrial RNA polymerase; this is the first ever-obtained evidence for an in vivo role of an animal mitochondrial transcription termination factor. In addition, the reported
data provide interesting insights into the involvement of DmTTF in transcription initiation in Drosophila mitochondria. (literal)
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