http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12749

Impaired mitochondrial glutamate transport in autosomal recessive neonatal myoclonic epilepsy (Articolo in rivista)

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Impaired mitochondrial glutamate transport in autosomal recessive neonatal myoclonic epilepsy (Articolo in rivista) (literal)

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Molinari F, Raas-Rothschild A, Rio M, Fiermonte G, Encha-Razavi F, Palmieri L, Palmieri F, Ben-Neriah Z, Kadhom N, Vekemans M, Attie-Bitach T, Munnich A, Rustin P, Colleaux L. (2005)
Impaired mitochondrial glutamate transport in autosomal recessive neonatal myoclonic epilepsy
in American journal of human genetics; Elsevier Inc., San Diego (Stati Uniti d'America)
(literal)

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Molinari F, Raas-Rothschild A, Rio M, Fiermonte G, Encha-Razavi F, Palmieri L, Palmieri F, Ben-Neriah Z, Kadhom N, Vekemans M, Attie-Bitach T, Munnich A, Rustin P, Colleaux L. (literal)

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Hospital Necker-Enfants Malades, Paris; 2Department of Human Genetics, Hadassah Hebrew University Medical Center, Jerusalem; and 3Department of Pharmaco-Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, and 4Consiglio Nazionale delle Richerche (CNR) Institute of Biomembranes and Bioenergetics, Bari, Italy (literal)

Titolo

Impaired mitochondrial glutamate transport in autosomal recessive neonatal myoclonic epilepsy (literal)

Abstract

Severe neonatal epilepsies with suppression-burst pattern are epileptic syndromes with either neonatal onset or onset during the first months of life. These disorders are characterized by a typical electroencephalogram pattern--namely, suppression burst, in which higher-voltage bursts of slow waves mixed with multifocal spikes alternate with isoelectric suppression phases. Here, we report the genetic mapping of an autosomal recessive form of this condition to chromosome 11p15.5 and the identification of a missense mutation (p.Pro206Leu) in the gene encoding one of the two mitochondrial glutamate/H(+) symporters (SLC25A22, also known as \"GC1\"). The mutation cosegregated with the disease and altered a highly conserved amino acid. Functional analyses showed that glutamate oxidation in cultured skin fibroblasts from patients was strongly defective. Further studies in reconstituted proteoliposomes showed defective [(14)C]glutamate uniport and [(14)C]glutamate/glutamate exchange by mutant protein. Moreover, expression studies showed that, during human development, SLC25A22 is specifically expressed in the brain, within territories proposed to contribute to the genesis and control of myoclonic seizures. These findings provide the first direct molecular link between glutamate mitochondrial metabolism and myoclonic epilepsy and suggest potential insights into the pathophysiological bases of severe neonatal epilepsies with suppression-burst pattern. (literal)

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