http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12617
Manganese Interferes with Calcium, Perturbs ERK Signaling, and Produces Embryos with No Skeleton. (Articolo in rivista)
- Type
- Label
- Manganese Interferes with Calcium, Perturbs ERK Signaling, and Produces Embryos with No Skeleton. (Articolo in rivista) (literal)
- Anno
- 2011-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1093/toxsci/kfr152 (literal)
- Alternative label
Pinsino A, Roccheri MC, Costa C, Matranga V. (2011)
Manganese Interferes with Calcium, Perturbs ERK Signaling, and Produces Embryos with No Skeleton.
in Toxicological sciences (Print)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Pinsino A, Roccheri MC, Costa C, Matranga V. (literal)
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- Rivista
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- CNR - IBIM - Istituto di Biomedicina e Immunologia Molecolare \"Alberto Monroy\" - Via Ugo La Malfa 153, 90146 Palermo - Italy (literal)
- Titolo
- Manganese Interferes with Calcium, Perturbs ERK Signaling, and Produces Embryos with No Skeleton. (literal)
- Abstract
- Manganese (Mn) has been associated with embryo toxicity as it
impairs differentiation of neural and skeletogenic cells in
vertebrates. Nevertheless, information on the mechanisms oper-
ating at the cellular level remains scant. We took advantage of an
amenable embryonic model to investigate the effects of Mn in
biomineral formation. Sea urchin (Paracentrotus lividus) embryos
were exposed to Mn from fertilization, harvested at different
developmental stages, and analyzed for their content in calcium
(Ca), expression of skeletogenic genes, localization of germ layer
markers, and activation of the extracellular signal-regulated kinase
(ERK). By optical and immunofluorescence microscopy, we found
that Mn exposure produced embryos with no skeleton, by
preventing the deposition of the triradiate calcitic spicules usually
produced only by specialized mesoderm cells. On the contrary,
ectoderm and endoderm differentiation was not impaired. Endog-
enous Ca content in whole embryos and its localization in Golgi
regions of skeletogenic cells was strongly reduced, as measured by
atomic absorption spectrometry and in vivo calcein labeling.
Spicule-lacking embryos showed persistent ERK activation by
immunocytochemistry and immunoblotting, contrary to the phys-
iological oscillations observed in normal embryos. The expression of
the skeletogenic genes, Pl-msp130 and Pl-sm30, was also differen-
tially affected if compared with controls. Here, we showed for the
first time the ability of Mn to interfere with Ca uptake and
internalization into skeletogenic cells and demonstrate that Ca
content regulates ERK activation/inactivation during sea urchin
embryomorphogenesis. The use ofMn-exposed sea urchin embryos
as a new model to study signaling pathways occurring during
skeletogenesis will provide new insights into the mechanisms
involved in Mn embryo toxicity and underlie the role of calcium in
the biomineralization process in vertebrates. (literal)
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