Toxicity of metal oxide nanoparticles in immune cells of the sea urchin (Articolo in rivista)

Type
Label
  • Toxicity of metal oxide nanoparticles in immune cells of the sea urchin (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Alternative label
  • Falugi C, Aluigi MG, Chiantore MC, Privitera D, Ramoino P, Gatti MA, Fabrizi A, Pinsino A, Matranga V (2012)
    Toxicity of metal oxide nanoparticles in immune cells of the sea urchin
    in Marine environmental research
    (literal)
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  • Falugi C, Aluigi MG, Chiantore MC, Privitera D, Ramoino P, Gatti MA, Fabrizi A, Pinsino A, Matranga V (literal)
Rivista
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  • accepted upon revision (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • Dipartimento per lo Studio del Territorio e delle sue Risorse, Università di Genova, Italy Laboratorio di Biomateriali, Università di Modena e Reggio Emilia, Italy Istituto di Biomedicina e Immunologia Molecolare Alberto Monroy, Consiglio Nazionale delle Ricerche, Palermo, Italy (literal)
Titolo
  • Toxicity of metal oxide nanoparticles in immune cells of the sea urchin (literal)
Abstract
  • The potential toxicity of stannum dioxide (SnO2), cerium dioxide (CeO2) and iron oxide (Fe3O4) nanoparticles (NPs) in the marine environment was investigated using the sea urchin,Paracentrotus lividus, as anin vivo model. We found that 5 days after force-feeding of NPs in aqueous solutions, the three NPs presented different toxicity degrees, depending on the considered biomarkers. We examined: 1) the presence of the NPs in the coelomicfluid and the uptake into the immune cells (coelomocytes); 2) the cholinesterase activity and the expression of the stress-related proteins HSC70 and GRP78; 3) the morphological changes affecting cellular compartments, such as the endoplasmic reticulum (ER) and lysosomes. By Environmental Scanning Electron Microscope (ESEM) analysis, coupled with Energy Dispersive X-ray Spectroscopy (EDS) we found that NPs were uptaken inside coelomocytes. The cholinesterases activity, a well known marker of blood intoxication in vertebrates, was greatly reduced in specimens exposed to NPs. We found that levels of stress proteins were down-regulated, matching the observed ER and lysosomes morphological alterations. In conclusion, this is thefirst study which utilizes the sea urchin as a model organism for biomonitoring the biological impact of NPs and supports the efficacy of the selected biomarkers. ? (literal)
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