Sea urchin embryos as an in vivo model for the assessment of manganese toxicity: developmental and stress response effects. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Sea urchin embryos as an in vivo model for the assessment of manganese toxicity: developmental and stress response effects. (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s10646-009-0432-0 (literal)

Alternative label

• Pinsino A, Matranga V, Trinchella F, Roccheri MC. (2010)
  Sea urchin embryos as an in vivo model for the assessment of manganese toxicity: developmental and stress response effects.
  in Ecotoxicology (Lond.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Pinsino A, Matranga V, Trinchella F, Roccheri MC. (literal)

Pagina inizio

• 555 (literal)

Pagina fine

• 562 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 19 (literal)

Rivista

• Ecotoxicology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dipartimento di Biologia Cellulare e dello Sviluppo ''A. Monroy'', Universita` di Palermo, Viale delle Scienze Ed 16, 90128 Palermo, Italia; Consiglio Nazionale delle Ricerche, Istituto di Biomedicina e Immunologia Molecolare ''Alberto Monroy'', Via Ugo La Malfa 153, 90146 Palermo, Italy; Dipartimento delle Scienze Biologiche, Universita` degli Studi di Napoli Federico II, Via Mezzocannone 8, 80134 Naples, Italy (literal)

Titolo

• Sea urchin embryos as an in vivo model for the assessment of manganese toxicity: developmental and stress response effects. (literal)

Abstract

• In the marine environment increasing concentrations of bio-available compounds often result from anthropogenic activities. Among metal ions, manganese represents a new emergent factor in environmental contamination. Here, we studied the effects of manganese on Paracentrotus lividus sea urchin embryos using biological and biochemical approaches for the analysis of impact on development, tissue accumulation and stress markers. Embryos were continuously exposed from fertilization to manganese at concentrations ranging from 1.0 to 61.6 mg l(-1), monitored for developmental abnormalities at 48 h after fertilization, and used for atomic spectrometric analysis at various times from 6 to 72 h. We found that concentration- and time-dependent increases in morphological abnormalities were directly correlated to manganese accumulation, with major defects in skeleton formation at 48 h. Concurrently, we found an upregulation of the hsc70 and hsc60 stress proteins detected by immunoblotting, whereas no induction of apoptosis or ROS production was observed by TUNEL and live tests, respectively. Taken together, our findings demonstrate that the observed manganese embryo-toxicity is related to both its intracellular accumulation and misregulated homeostasis, and confirm the importance of stress proteins as protective agents in the acquisition of tolerance and resistance to apoptosis. (literal)

Prodotto di

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• Stress Cellulare ed Ambiente (SV.P17.008.003) (Modulo)

Autore CNR

• VALERIA MATRANGA (Unità di personale interno)
• ANNALISA PINSINO (Persona)

Insieme di parole chiave

• Keywords of "Sea urchin embryos as an in vivo model for the assessment of manganese toxicity: developmental and stress response effects." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• VALERIA MATRANGA (Unità di personale interno)
• ANNALISA PINSINO (Persona)

Prodotto

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• Stress Cellulare ed Ambiente (SV.P17.008.003) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Ecotoxicology (Rivista)

Insieme di parole chiave di

• Keywords of "Sea urchin embryos as an in vivo model for the assessment of manganese toxicity: developmental and stress response effects." (Insieme di parole chiave)