http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12536

Myc promoter-binding protein-1 (MBP-1) is a novel potential prognostic marker in invasive ductal breast carcinoma. (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Myc promoter-binding protein-1 (MBP-1) is a novel potential prognostic marker in invasive ductal breast carcinoma. (Articolo in rivista) (literal)

Anno

2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1371/journal.pone.0012961 (literal)

Alternative label

Lo Presti M; Ferro A; Contino F; Mazzarella C; Sbacchi S; Roz E; Lupo C; Perconti G; Giallongo A; Migliorini P; Marrazzo A; Feo S (2010)
Myc promoter-binding protein-1 (MBP-1) is a novel potential prognostic marker in invasive ductal breast carcinoma.
in PloS one; Public Library of Science, San Francisco (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Lo Presti M; Ferro A; Contino F; Mazzarella C; Sbacchi S; Roz E; Lupo C; Perconti G; Giallongo A; Migliorini P; Marrazzo A; Feo S (literal)

Pagina inizio

1 (literal)

Pagina fine

10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

5 (literal)

Rivista

PloS one (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

Sep 23;5(9):e12961. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

9 (literal)

Note

PubMe (literal)
ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Dipartimento di Oncologia Sperimentale e Applicazioni Cliniche, Università di Palermo, Palermo, Italy Istituto di Biomedicina e Immunologia Molecolare, CNR, Palermo, Italy Dipartimento di Medicina Interna, Università di Pisa, Pisa, Italy Dipartimento Oncologico di III livello La Maddalena, Palermo, Italy (literal)

Titolo

Myc promoter-binding protein-1 (MBP-1) is a novel potential prognostic marker in invasive ductal breast carcinoma. (literal)

Abstract

Background: Alpha-enolase is a glycolytic enzyme that catalyses the formation of phosphoenolpyruvate in the cell cytoplasm. a-Enolase and the predominantly nuclear Myc promoter-binding protein-1 (MBP-1) originate from a single gene through the alternative use of translational starting sites. MBP-1 binds to the P2 c-myc promoter and competes with TATAbox binding protein (TBP) to suppress gene transcription. Although several studies have shown an antiproliferative effect of MBP-1 overexpression on several human cancer cells, to date detailed observations of a-enolase and MBP-1 relative expression in primary tumors versus normal tissues and their correlation with clinicopathological features have not been undertaken. Methodology and Findings: We analyzed a-enolase and MBP-1 expression in normal breast epithelium and primary invasive ductal breast carcinoma (IDC) from 177 patients by Western blot and immunohistochemical analyses, using highly specific anti-a-enolase monoclonal antibodies. A significant increase in the expression of cytoplasmic a-enolase was observed in 98% of the tumors analysed, compared to normal tissues. Nuclear MBP-1 was found in almost all the normal tissues while its expression was retained in only 35% of the tumors. Statistically significant associations were observed among the nuclear expression of MBP-1 and ErbB2 status, Ki-67 expression, node status and tumor grade. Furthermore MBP-1 expression was associated with good survival of patients with IDC. Conclusions: MBP-1 functions in repressing c-myc gene expression and the results presented indicate that the loss of nuclear MBP-1 expression in a large number of IDC may be a critical step in the development and progression of breast cancer and a predictor of adverse outcome. Nuclear MBP-1 appears to be a novel and valuable histochemical marker with potential prognostic value in breast cancer. (literal)

Editore