http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12495
Transcriptional increase and misexpression of 14-3-3 epsilon in sea urchin embryos exposed to UV-B. (Articolo in rivista)
- Type
- Label
- Transcriptional increase and misexpression of 14-3-3 epsilon in sea urchin embryos exposed to UV-B. (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s12192-010-0210-1 (literal)
- Alternative label
Russo R, Zito F, Costa C, Bonaventura R, Matranga V. (2010)
Transcriptional increase and misexpression of 14-3-3 epsilon in sea urchin embryos exposed to UV-B.
in Cell stress & chaperones
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Russo R, Zito F, Costa C, Bonaventura R, Matranga V. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- 2010 Jul 4. [Epub ahead of print] PubMed PMID: 20607471. (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Consiglio Nazionale delle Ricerche, Istituto di Biomedicina e Immunologia Molecolare, \"Alberto Monroy\", Via Ugo La Malfa 153, 90146 Palermo, Italy (literal)
- Titolo
- Transcriptional increase and misexpression of 14-3-3 epsilon in sea urchin embryos exposed to UV-B. (literal)
- Abstract
- Members of the 14-3-3 protein family are involved in many important cellular events, including stress response, survival and apoptosis. Genes of the 14-3-3 family are conserved from plants to humans, and some members are responsive to UV radiation. Here, we report the isolation of the complete cDNA encoding the 14-3-3 epsilon isoform from Paracentrotus lividus sea urchin embryos, referred to as Pl14-3-3 epsilon, and the phylogenetic relationship with other homologues described in different phyla. Pl14-3-3 epsilon mRNA levels were measured by QPCR during development and found to increase from the mesenchyme blastula to the prism stage. In response to UV-B (312 nm) exposure, early stage embryos collected 2 h later showed a 2.3-fold (at 400 J/m(2)) and a 2.7-fold (at 800 J/m(2)) increase in Pl14-3-3 epsilon transcript levels compared with controls. The spatial expression of Pl14-3-3 epsilon mRNA, detected by whole mount in situ hybridization in both control and UV-B exposed embryos, harvested at late developmental stages, showed transcripts to be located in the archenteron of gastrula stage and widely distributed in all germ layers, respectively. The Pl14-3-3 epsilon mRNA delocalization parallels the failure in archenteron elongation observed morphologically, as well as the lack of specific endoderm markers, investigated by indirect immuno-fluorescence on whole mount embryos. Results confirm the involvement of 14-3-3 epsilon in the stress response elicited by UV-B and demonstrate, for the first time, its contribution at the transcriptional level in the sea urchin embryo (literal)
- Prodotto di
- Autore CNR
Incoming links:
- Autore CNR di
- Prodotto
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi