http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12251
Variable p-CREB expression depicts different asthma phenotypes (Articolo in rivista)
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- Label
- Variable p-CREB expression depicts different asthma phenotypes (Articolo in rivista) (literal)
- Anno
- 2007-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1111/j.1398-9995.2007.01417.x (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- G. Chiappara1; P. Chanez2; A. Bruno1; E. Pace1; F. Pompeo1; J. Bousquet2; G. Bonsignore1;
M. Gjomarkaj1 (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1 Istituto di Biomedicina e Immunologia Molecolare,Commessa di Immunopatologia e Farmacologia Sperimentale dell'Appartato Respiratorio, Consiglio Nazionale delle Ricerche, Palermo, Italy; 2 Clinique des Maladies Respiratoires, INSERM-U454, CHU Montpellier, France (literal)
- Titolo
- Variable p-CREB expression depicts different asthma phenotypes (literal)
- Abstract
- Background: Chromatin modification may play a role in inflammatory gene regulation in asthma. Cyclic adenosine mono-phosphate response element binding protein (CREB), with the specific co-activator, the CREB-binding protein (CBP), contributes to the acetylation of chromatin and to the transcription of pro-inflammatory genes.
Objectives: To evaluate the expression of CBP and of phospho-CREB (p-CREB) in bronchial biopsies and in peripheral blood mononuclear cells (PBMC) of controls (C), untreated (UA), inhaled steroid treated (ICS) and steroiddependent asthmatic (SDA) patients.
Methods: We used immunohistochemistry in bronchial biopsies and western blot analysis and immunocytochemistry in PBMC.
Results: Cyclic adenosine mono-phosphate response element-binding protein expression, in the epithelium was similar in all groups, while p-CREB expression was increased in UA and in SDA in comparison with ICS and C subjects (C vs UA P = 0.002, C vs SDA P = 0.007), (ICS vs SDA P = 0.005), (ICS vs UA P = 0.001). Interestingly, also in the submucosa, p-CREB was increased in UA
and SDA in comparison with ICS and C subjects (C vs UA P = 0.0004) (C vs SDA P < 0.0001) (ICS vs UA P = 0.002) (ICS vs SDA P < 0.0001) and positively correlated with leukocyte infiltration within the bronchi (CD45RB+cells). Similar results were obtained with PBMC isolated from the same patient groups. Incubation of PBMC in vitro, with fluticasone propionate, decreased the p-CREB expression induced by cytokine activation (interferon-c, tumor necrosis
factor-a). Conclusions: This study demonstrates that the expression of p-CREB is related,
in asthma, to the persistent inflammation according to the disease severity.
p-CREB expression can be modulated by glucocorticoids in responsive patients. (literal)
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