http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12247
Comparative study of T84 and T84SF human colon carcinoma cells: in vitro and in vivo ultrastructural and functional characterization of cell culture and metastasis (Articolo in rivista)
- Type
- Label
- Comparative study of T84 and T84SF human colon carcinoma cells: in vitro and in vivo ultrastructural and functional characterization of cell culture and metastasis (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s00428-006-0179-4 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Alessandro R.; Di Bella M.A.; Flugy A.M.; Fontana S.; Damiani F.; Corrado C.; Colomba P.; Todaro M.; Russo D.; Santoro A.; Kohn E.C.; De Leo G; (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- R. Alessandro (*) . M. A. Di Bella . A. M. Flugy . S. Fontana .
F. Damiani . C. Corrado . P. Colomba . G. De Leo
Dipartimento di Biopatologia e Metodologie Biomediche,
Sezione di Biologia e Genetica, Università di Palermo,
Via Divisi 83,
90133 Palermo, Italy
e-mail: ricale@unipa.it
Tel.: +39-091-6554600
Fax: +39-091-6554624
M. Todaro
Dipartimento di Discipline Chirurgiche ed Oncologiche,
Università di Palermo,
Palermo, Italy
D. Russo
Istituto di Biomedicina ed Immunologia
Molecolare \"Alberto Monroy\", Centro Nazionale delle
Ricerche,
Palermo, Italy
A. Santoro
Laboratorio di Ematologia,
Dipartimento di Ricerca Clinica e Biotecnologia,
A.O. \"V.Cervello\",
Palermo, Italy
E. C. Kohn
Molecular Signalling Section, Laboratory of Pathology,
Center for Cancer Research, National Cancer Institute,
Bethesda, MD, USA (literal)
- Titolo
- Comparative study of T84 and T84SF human colon carcinoma cells: in vitro and in vivo ultrastructural and functional characterization of cell culture and metastasis (literal)
- Abstract
- Abstract To better understand the relationship between
tumor heterogeneity, differentiation, and metastasis, suitable
experimental models permitting in vitro and in vivo
studies are necessary. A new variant cell line (T84SF)
exhibiting an altered phenotype was recently selected from
a colon cancer cell line (T84) by repetitive plating on
TNF-? treated human endothelial cells and subsequent
selection for adherent cells. The matched pair of cell lines
provides a useful system to investigate the extravasation
step of the metastatic cascade. Since analysis of morphological
differences can be instructive to the understanding
of metastatic potential of tumor cells, we compared the
ultrastructural and functional phenotype of T84 and T84SF
cells in vitro and in vivo. The reported ultrastructural
features evidence differences between the two cell lines;
selected cells showed a marked pleomorphism of cell size
and nuclei, shape, and greater surface complexity. These
morphological differences were also coupled with biochemical
data showing a distinct tyrosine phosphorylation-
based signaling, an altered localization of ?-catenin,
MAPK, and AKT activation, as well as an increased expression
in T84SF cells of Bcl-XL, a major regulator of
apoptosis. Therefore, these cell lines represent a step
forward in the development of appropriate models in vitro
and in vivo to investigate colon cancer progression. (literal)
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