http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12228
Identification and phenotypic characterization of a subpopulation of T84 human colon cancer cells, after selection on activated endothelial cells (Articolo in rivista)
- Type
- Label
- Identification and phenotypic characterization of a subpopulation of T84 human colon cancer cells, after selection on activated endothelial cells (Articolo in rivista) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1002/jcp.20236 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- R. ALESSANDRO;1* A.M. FLUGY;1 D. RUSSO;2 G. STASSI;3 A. DE LEO;4 C. CORRADO;1
G. ALAIMO;1
AND G. DE LEO1 (literal)
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- 1Dipartimento di Biopatologia e Metodologie Biomediche,
Universita` di Palermo, Palermo, Italy
2Istituto di Biomedicina ed Immunologia Molecolare ''Alberto Monroy'', Centro
Nazionale delle Ricerche, Palermo, Italy
3Dipartimento di Discipline Chirurgiche ed Oncologiche,
Universita` di Palermo, Palermo, Italy
4Dipartimento di Malattie Cutanee, Veneree,
e Chirurgia Plastica Ricostruttiva, Universita` di Roma ''La Sapienza'', Rome, Italy (literal)
- Titolo
- Identification and phenotypic characterization of a subpopulation of T84 human colon cancer cells, after selection on activated endothelial cells (literal)
- Abstract
- The extravasation of metastatic cells is regulated by molecular events involving the initial adhesion of tumor cells to the endothelium
and subsequently the migration of the cells in the host connective tissue. The differences in metastatic ability could be attributed to
properties intrinsic of the various primary tumor types. Thus, the clonal selection of neoplastic cells during cancer progression
results in cells better equipped for survival and formation of colonies in secondary sites. A cell line (T84SF) exhibiting an altered
phenotypic appearance was selected from a colon cancer cell line (T84) by repetitive plating on TNFa-activated human endothelial
cells and subsequent selection for adherent cells. Cell growth, motility, chemoinvasive abilities, tyrosine phosphorylation signaling,
and the metastasis formation in nude mice of the two cell lines was compared. T84SF cells displayed in vitro an higher proliferation
rate and a more invasive behavior compared to the parental cells while formed in vivo a greater number of metastatic colonies in
nude mice. As concerns the signaling underlying the phenotypes of the selected cells, we examined the general tyrosine phosphorylation
levels in both cell lines. Our results indicate that T84SF have an increased basal tyrosine phosphorylation of several
proteins among which src kinase was identified. Treatment of cells with a specific inhibitor of src activity caused a greater in vitro
inhibition of proliferation and invasive properties of T84 parental cells with respect to T84SF cells and diminished metastasis
formation in vivo. Altogether, these data provide evidences that this new cell line may be valuable for identifying molecular
mechanisms involved in the metastatic progression of colon cancer. J. Cell. Physiol. 203: 261-272, 2005. ? 2004 Wiley-Liss, Inc. (literal)
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