http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12226
Seawi--a sea urchin piwi/argonaute family member is a component of MT-RNP complexes (Articolo in rivista)
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- Seawi--a sea urchin piwi/argonaute family member is a component of MT-RNP complexes (Articolo in rivista) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
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- Rodriguez A.J.; Seipel S.A.; Hamill D.R.; Romancino D.P.; DI Carlo M.; Suprenant K.A.; Bonder E.M. (literal)
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- ISI Web of Science (WOS) (literal)
- Scopus (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Titolo
- Seawi--a sea urchin piwi/argonaute family member is a component of MT-RNP complexes (literal)
- Abstract
- The piwi/argonaute family of proteins is involved in key developmental processes such as stem cell maintenance and axis
specification through molecular mechanisms that may involve RNA silencing. Here we report on the cloning and characterization
of the sea urchin piwi/argonaute family member seawi. Seawi is a major component of microtubule-ribonucleoprotein
(MT-RNP) complexes isolated from two different species of sea urchin, Strongylocentrotus purpuratus and Paracentrotus lividus.
Seawi co-isolates with purified ribosomes, cosediments with 80S ribosomes in sucrose density gradients, and binds microtubules.
Seawi possesses the RNA binding motif common to piwi family members and binds P. lividus bep4 mRNA, a transcript
that co-isolates with MT-RNP complexes and whose translation product has been shown to play a role in patterning the
animal-vegetal axis. Indirect immunofluorescence studies localized seawi to the cortex of unfertilized eggs within granule-like
particles, the mitotic spindle during cell division, and the small micromeres where its levels were enriched during the early
cleavage stage. Lastly, we discuss how seawi, as a piwi/argonaute family member, may play a fundamentally important role in
sea urchin animal-vegetal axis formation and stem cell maintenance. (literal)
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