http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12209
Biochemical interaction between effects of beclomethasone dipropionate and salbutamol or formoterol in sputum cells from mild to moderate asthmatics (Articolo in rivista)
- Type
- Label
- Biochemical interaction between effects of beclomethasone dipropionate and salbutamol or formoterol in sputum cells from mild to moderate asthmatics (Articolo in rivista) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1111/j.1398-9995.2005.00702.x (literal)
- Alternative label
Profita M., 1; Gagliardo R., 1; Di Giorgi R., 1; Pompeo F., 1; Gjomarkaj M., 1; Nicolini G., 2; Bousquet J., 3; Vignola AM., 1. (2005)
Biochemical interaction between effects of beclomethasone dipropionate and salbutamol or formoterol in sputum cells from mild to moderate asthmatics
in Allergy (Cph.)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Profita M., 1; Gagliardo R., 1; Di Giorgi R., 1; Pompeo F., 1; Gjomarkaj M., 1; Nicolini G., 2; Bousquet J., 3; Vignola AM., 1. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1 Istituto di biomedicina e immunologia Molecolare \"Alberto Monroy\";
2 Chiesi Farmaceutici;
3 INSERMU454, Montpellier, France (literal)
- Titolo
- Biochemical interaction between effects of beclomethasone dipropionate and salbutamol or formoterol in sputum cells from mild to moderate asthmatics (literal)
- Abstract
- Background: Several in vitro studies demonstrate that corticosteroids and
long-acting b2 agonists may have a complementary and synergistic mode of
action on the inflammatory processes in asthma.
Methods: Sputum was induced in 20 mild to moderate asthmatic patients and the
induced sputum cells (ISC) were cultured with beclomethasone dipropionate
(BDP) 10)7 M, salbutamol 10)8 M and formoterol 10)8 M either alone or in
combination, BDP plus salbutamol and BDP plus formoterol, for 24 h. We
measured the levels of growth macrophages-colony stimulating factor
(GM-CSF), released on activation normal T cells expressed and activated
(RANTES) and interleukin-8 (IL-8), in the supernatant of stimulated cells by
ELISA. Furthermore, we assessed nuclear translocation of glucocorticoid
receptor (GR) and the expression of b2 receptor in ISC by immunofluorescence
and RT-PCR, respectively.
Results: The release of GM-CSF, RANTES and IL-8 in ISC was significantly
reduced by BDP plus salbutamol or formoterol as compared with either drug
alone (P < 0.0001). b2 receptor expression was increased after 30 min of incubation
with BDP and continued to increase over a time period of 4 h
(P < 0.0001). Furthermore after 30 min of incubation, nuclear translocation of
GR was greater with BDP plus salbutamol or formoterol than with any of the
drugs alone (P < 0.0001).
Conclusion: The present ex vivo study demonstrates a complementary mode
of action between BDP and salbutamol or formoterol leading to an enhanced
anti-inflammatory activity. (literal)
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