Biochemical interaction between effects of beclomethasone dipropionate and salbutamol or formoterol in sputum cells from mild to moderate asthmatics (Articolo in rivista)

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Label
  • Biochemical interaction between effects of beclomethasone dipropionate and salbutamol or formoterol in sputum cells from mild to moderate asthmatics (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1111/j.1398-9995.2005.00702.x (literal)
Alternative label
  • Profita M., 1; Gagliardo R., 1; Di Giorgi R., 1; Pompeo F., 1; Gjomarkaj M., 1; Nicolini G., 2; Bousquet J., 3; Vignola AM., 1. (2005)
    Biochemical interaction between effects of beclomethasone dipropionate and salbutamol or formoterol in sputum cells from mild to moderate asthmatics
    in Allergy (Cph.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Profita M., 1; Gagliardo R., 1; Di Giorgi R., 1; Pompeo F., 1; Gjomarkaj M., 1; Nicolini G., 2; Bousquet J., 3; Vignola AM., 1. (literal)
Pagina inizio
  • 323 (literal)
Pagina fine
  • 329 (literal)
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  • 60 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 3 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 Istituto di biomedicina e immunologia Molecolare \"Alberto Monroy\"; 2 Chiesi Farmaceutici; 3 INSERMU454, Montpellier, France (literal)
Titolo
  • Biochemical interaction between effects of beclomethasone dipropionate and salbutamol or formoterol in sputum cells from mild to moderate asthmatics (literal)
Abstract
  • Background: Several in vitro studies demonstrate that corticosteroids and long-acting b2 agonists may have a complementary and synergistic mode of action on the inflammatory processes in asthma. Methods: Sputum was induced in 20 mild to moderate asthmatic patients and the induced sputum cells (ISC) were cultured with beclomethasone dipropionate (BDP) 10)7 M, salbutamol 10)8 M and formoterol 10)8 M either alone or in combination, BDP plus salbutamol and BDP plus formoterol, for 24 h. We measured the levels of growth macrophages-colony stimulating factor (GM-CSF), released on activation normal T cells expressed and activated (RANTES) and interleukin-8 (IL-8), in the supernatant of stimulated cells by ELISA. Furthermore, we assessed nuclear translocation of glucocorticoid receptor (GR) and the expression of b2 receptor in ISC by immunofluorescence and RT-PCR, respectively. Results: The release of GM-CSF, RANTES and IL-8 in ISC was significantly reduced by BDP plus salbutamol or formoterol as compared with either drug alone (P < 0.0001). b2 receptor expression was increased after 30 min of incubation with BDP and continued to increase over a time period of 4 h (P < 0.0001). Furthermore after 30 min of incubation, nuclear translocation of GR was greater with BDP plus salbutamol or formoterol than with any of the drugs alone (P < 0.0001). Conclusion: The present ex vivo study demonstrates a complementary mode of action between BDP and salbutamol or formoterol leading to an enhanced anti-inflammatory activity. (literal)
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