http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12190
Paracentrotus lividus eggs contain different RNAs at the animal and vegetal pole (Articolo in rivista)
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- Label
- Paracentrotus lividus eggs contain different RNAs at the animal and vegetal pole (Articolo in rivista) (literal)
- Anno
- 2004-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/jbbrc.2004.01.167 (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Di Carlo M.; Montana G.; Romancino D.P. (literal)
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- Scopu (literal)
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Titolo
- Paracentrotus lividus eggs contain different RNAs at the animal and vegetal pole (literal)
- Abstract
- Paracentrotus lividus eggs were divided by centrifugation into nucleated and anucleated halves. Fertilization and development of
the two halves permitted us to establish that nucleated and anucleated fragments correspond, respectively, to the animal and vegetal
parts. RNA was extracted from both egg halves and submitted to differential display. Northern blot analysis confirmed their
maternal origin and showed that each transcript has a different expression pattern during development. By Northern blot and in situ
hybridization experiments we ascertained that Bep2 and PlAn1 are localized in the animal part, whereas 16S rRNA, Plveg1, and L27
in the vegetal part, and that Plun1 is uniformly distributed. Moreover, by treating P. lividus eggs with detergent, in presence or not of
drugs such as colchicine and cytochalasin B, we demonstrated the involvement of the cytoskeleton only in localization of Bep2,
PlAn1, and Plun1, suggesting that different mechanisms are utilized for animal and vegetal distribution. (literal)
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