http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12145
17b-estradiol synthesis in the adult male rat retina. (Articolo in rivista)
- Type
- Label
- 17b-estradiol synthesis in the adult male rat retina. (Articolo in rivista) (literal)
- Anno
- 2007-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.exer.2007.02.008 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- C. Cascio; D. Russo; G. Drago; G. Galizzi; R. Passantino; R. Guarneri; P. Guarneri (literal)
- Pagina inizio
- Pagina fine
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- Prodotto digitalizzato (literal)
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- Rivista
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" CNR Palermo Italy (literal)
- Titolo
- 17b-estradiol synthesis in the adult male rat retina. (literal)
- Abstract
- 17b-Estradiol (E2) exerts neurotrophic and neuroprotective effects in the retina as well as in other CNS structures, independently of sex. Retinal
effects, however, have not been supported by evidence on local synthesis, and whether CNS 17b-estradiol is formed in a neurosteroidogenic
pathway starting from cholesterol conversion into pregnenolone is a question still left unanswered. In the adult male rat retina, we have previously
showed localization and activity of the P450 side chain cleavage (P450scc) enzyme, which is involved in pregnenolone synthesis.
Here, we demonstrate both the mRNA and protein expression of 3b-hydroxysteroid dehydrogenase (3b-HSD), P450aromatase and also of
P450scc, but only the protein expression of P450 17a-hydroxylase/lyase (P450c17). Using radiolabeled pregnenolone and testosterone as precursors,
in the isolated and intact retina of adult male rats, E2 is produced in a large amount by each precursor within 1e4 h, suggesting a highly
active metabolic pathway towards its formation. The immunolocalization pattern shows enzymes and estrogen receptor subtypes (ERa, ERb)
scattered in the retina with different intensities throughout the layers. The results point to the adult male rat retina as a neurosteroidogenic structure
where E2 synthesis via a progesterone pathway and the presence of estrogen receptors provide important clues for understanding the neurotrophic
and neuroprotective effects of the steroid hormone. (literal)
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