CTLA-4 REGULATES ALLERGEN RESPONSE BY MODULATING GATA-3 PROTEIN LEVEL/CELL\" (Articolo in rivista)

Type
Label
  • CTLA-4 REGULATES ALLERGEN RESPONSE BY MODULATING GATA-3 PROTEIN LEVEL/CELL\" (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1111/j.1365-2567.2007.02537.x (literal)
Alternative label
  • 1 Francesca Nasta; 2 Silvia Corinti; 3 Angela Bonura; 3 Paolo Colombo; 2 Gabriella Di Felice and 1 Claudio Pioli. (2007)
    CTLA-4 REGULATES ALLERGEN RESPONSE BY MODULATING GATA-3 PROTEIN LEVEL/CELL"
    in Immunology (Oxf., Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • 1 Francesca Nasta; 2 Silvia Corinti; 3 Angela Bonura; 3 Paolo Colombo; 2 Gabriella Di Felice and 1 Claudio Pioli. (literal)
Pagina inizio
  • 62 (literal)
Pagina fine
  • 70 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 121 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 9 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 ENEA, Unit of Biotechnology, Section of Toxicology and Biomedicine, Rome, Italy,; 2 ISS, Unit of Immune-mediated Diseases,Department of Infectious, Parasitic and Immune-mediated Diseases, Rome; 3CNR, Institute of Biomedicine andMolecular Immunology, Palermo, Italy. (literal)
Titolo
  • CTLA-4 REGULATES ALLERGEN RESPONSE BY MODULATING GATA-3 PROTEIN LEVEL/CELL\" (literal)
Abstract
  • T helper type 2 (Th2) cell differentiation requires the expression of GATA-3, a transcription factor that allows transcriptional activation of Th2 cytokine genes through chromatin remodelling. We investigated the role of the negative costimulatory receptor cytotoxic T-lymphocyte antigen 4 (CTLA-4) in the regulation of GATA-3 expression, Th2 differentiation and immunoglobulin production during the immune response to allergens. BALB/c mice were immunized with a recombinant major allergenic component of Parietaria judaica pollen, rPar j I, and treated with blocking anti-CTLA-4 or control antibodies. Results showed that in vivo CTLA-4 blockade enhanced the Par j I-specific immunoglobulin E (IgE) serum level. In contrast, Par j I-specific IgG2a serum level was reduced, suggesting that CTLA-4 blockade skewed immunoglobulin production towards interleukin-4 (IL-4) -dependent immunoglobulin isotypes. Consistently, CTLA-4 blockade increased the frequency of Par j I-specific Th2 cells but not Th1 cells, as well as IL-4 and IL-5 but not interferon-c production. Our data also showed that CTLA-4 blockade enhanced the GATA-3 : T-bet messenger RNA ratio. Interestingly, in vivo CTLA-4 blockade did not increase the frequency of GATA-3 protein-expressing cells. In contrast, it enhances GATA-3 protein level per cell. Further, in vitro results show that the anti-CTLA-4 monoclonal antibody, by competing with CD80 for CTLA-4 binding, induced an enhancement in the frequency of IL-4-producing cells that correlates with the increase in GATA-3 protein level per cell. In conclusion, CTLA-4, by affecting the level of GATA-3 per cell, contributes to keeping this factor under the threshold required to become a Th2 effector cell. Consequently, it affects IgE/IgG2a production and contributes to the outcome of allergen-specific immune responses. (literal)
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