http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12091
Toxicity of recombinant beta-amyloid prefibrillar oligomers on the morphogenesis of the sea urchin Paracentrotus lividus. (Articolo in rivista)
- Type
- Label
- Toxicity of recombinant beta-amyloid prefibrillar oligomers on the morphogenesis of the sea urchin Paracentrotus lividus. (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
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- Carrotta R.; Di Carlo M.; Manno M.; Montana G.; Picone P.; Romancino D.; San Biagio P.L. (literal)
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- Rivista
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- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Titolo
- Toxicity of recombinant beta-amyloid prefibrillar oligomers on the morphogenesis of the sea urchin Paracentrotus lividus. (literal)
- Abstract
- A distinctive feature of Alzheimer's disease
is the deposition of amyloid ?-protein (A?) in senile
or diffuse plaques. The 42 residue ?-peptide (A?42) is
the predominant form found in plaques. In the present
work we report a high-yield expression and purification
method of production of a recombinant A?42. The
purified recombinant peptide shows characteristics similar
to the synthetic human peptide. Different size aggregates,
either small oligomers or larger aggregates, were
obtained upon dissolving the recombinant A?42 peptide
under different conditions at pH 7.2 or pH 3, respectively.
We report a new toxicity assay on the morphogenic
development of the sea urchin Paracentrotus lividus and
study the toxicity of the two kinds of aggregates. Despite
the difference between the ionic strength of human
extracellular fluid (0.154 mol/l) and artificial sea water
(0.48 mol/l), toxicity data collected in this system have an
intrinsic relevance. The different ionic strength, in fact,
could change the kinetics of oligomer formation, but the
effect of morphogenic development reported here is
related to the final oligomer sizes. Results of the toxicity
assay of A?42 on sea urchin development also show a
dose-dependent effect. After only 4 h of embryo development,
one can note morphological defects in the cell
membrane. Retardation of the embryo's development,
along with cellular disorders visible inside the blastocoele,
can be observed after 1 day of development. Cellular
degeneration in two different pathological phenotypes--
the occluded blastulae and the occluded prism--is
present after 48 h of development. Results show that a
greater effect on cell death is induced by the small
oligomers stabilized under physiological conditions than
at acid pH. In this case only occluded blastulae are found
after 48 h of development. (literal)
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