http://www.cnr.it/ontology/cnr/individuo/prodotto/ID11975
Increased prostaglandin E2 concentrations and cyclooxygenase-2 expression in asthmatis subjects with sputum eosinophilia (Articolo in rivista)
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- Label
- Increased prostaglandin E2 concentrations and cyclooxygenase-2 expression in asthmatis subjects with sputum eosinophilia (Articolo in rivista) (literal)
- Anno
- 2003-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1067/mai.2003.1737 (literal)
- Alternative label
Profita M, Sala A, Bonanno A, Riccobono L, Siena L, Melis MR, Di Giorgi R, Mirabella F, Gjomarkaj M, Bonsignore G, Vignola AM. (2003)
Increased prostaglandin E2 concentrations and cyclooxygenase-2 expression in asthmatis subjects with sputum eosinophilia
in Journal of allergy and clinical immunology
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Profita M, Sala A, Bonanno A, Riccobono L, Siena L, Melis MR, Di Giorgi R, Mirabella F, Gjomarkaj M, Bonsignore G, Vignola AM. (literal)
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- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- Impact Factor: 5.560 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
- Il lavoro dimostra che, in soggetti asmatici, il fenomeno infiammatorio dell'albero bronchiale caratterizzato prevalentemente da una aumentata presenza di eosinofili é legato ad una più lunga sopravvivenza di queste cellule. I macrofagi alveolari esprimono COX2 e contribuiscono ad incrementare la sopravvivenza degli eosinofili mediante la produzione di PGE2. (literal)
- Note
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Angelo Sala:professore associato del Centro di Farmacologia Polmonare dell'Università degli Studi di Palermo, Vignola AM - Istituto di Medicina Generale e Pneumologia - Università di Palermo - Responsabile Sezione IFC-IBIM-CNR (literal)
- Titolo
- Increased prostaglandin E2 concentrations and cyclooxygenase-2 expression in asthmatis subjects with sputum eosinophilia (literal)
- Abstract
- BACKGROUND: Prostaglandin E2 (PGE2) is known to be produced within human
airways, but it is not clear whether in airway diseases it can play a
deleterious or a beneficial role. Recently it has been reported that PGE2 can
enhance eosinophil survival in vitro. OBJECTIVE: To evaluate whether the
concentrations of PGE2 in asthmatic airways correlate with the number of
eosinophils and can be responsible for eosinophil-enhanced survival and to
identify the cyclooxygenase isoform contributing to the synthesis of PGE2 by
cells present in asthmatic airways. METHODS: Reversed-phase high-performance
liquid chromatography and/or specific radioimmunoassay was used to measure PGE2
concentrations in induced sputum supernatants from 14 control and 30 asthmatic
subjects. Correlations between concentrations of PGE2 and the number of
eosinophils in induced sputum were evaluated. Expression of cyclooxygenase-2
(COX-2) in induced sputum cells was determined by immunocytochemistry, and the
effect of COX-2 inhibition on PGE2 production was evaluated with the use of
radiolabeled arachidonic acid. The effects on eosinophil apoptosis by PGE2 or
induced sputum supernatants were studied by using peripheral blood eosinophils
obtained by negative immunomagnetic selection. RESULTS: PGE2 concentrations
resulted in elevated samples from asthmatic subjects and directly correlated
with the percentage of eosinophils and the concentrations of eosinophilic
cationic protein. Immunostaining for COX-2 showed enhanced expression in
macrophages of asthmatic subjects when compared with control subjects, and the
use of a specific COX-2 inhibitor provided evidence that PGE2 synthesis was the
result of COX-2 enzymatic activity in asthma-induced sputum cells. Supernatant
from induced sputum of asthmatic subjects with high eosinophil counts caused a
decreased apoptosis of peripheral blood eosinophils when compared with control
subjects, and immunoprecipitation of PGE2 significantly reverted this
phenomenon, suggesting that PGE2 was present in biologically relevant
concentrations in induced sputum. CONCLUSIONS: The results obtained suggest that
COX-2 expression in alveolar macrophages from asthmatic subjects may contribute
to enhanced eosinophil survival through an increased PGE2 production. (literal)
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