Differences between brain structures in nuclear translocation and DNA binding of the glucocorticoid receptor during stress and the circadian cycle (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Differences between brain structures in nuclear translocation and DNA binding of the glucocorticoid receptor during stress and the circadian cycle (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Alternative label

• Kitchener P; Di Blasi F.; Borrelli E; Piazza PV. (2004)
  Differences between brain structures in nuclear translocation and DNA binding of the glucocorticoid receptor during stress and the circadian cycle
  in European journal of neuroscience (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Kitchener P; Di Blasi F.; Borrelli E; Piazza PV. (literal)

Pagina inizio

• 1837 (literal)

Pagina fine

• 1846 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 19 (literal)

Rivista

• European journal of neuroscience (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 7 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• INSERM U588, Laboratoire de Physiopathologie du Comportement, Université Bordeaux 2, Domaine de carreire, rue Camille Saint Saëns, 33077 Bordeaux Cedex, France. Istituto di Biomedicina e di Immunologia Molecolare \" Alberto Monroy\" - Consiglio Nazionale delle Ricerche Via Ugo La Malfa 153 90146 Palermo Italy Institut de Génétique et de Biologie Moléculaire et Cellulaire INSERM CNRS 1 Rue Laurent Fries 67400 Illkirch-Graffenstaden France (literal)

Titolo

• Differences between brain structures in nuclear translocation and DNA binding of the glucocorticoid receptor during stress and the circadian cycle (literal)

Abstract

• Glucocorticoid receptors (GRs) are transcription factors that, upon activation by glucocorticoids, translocate to the cell nucleus, and bind to specific response elements (GREs) in the promoter region of target genes. We analysed stress- and circadian-induced changes in nuclear translocation and GRE binding of GRs in the hippocampus and the prefrontal cortex of the rat brain. Nuclear translocation and binding to GRE were measured in nuclear extracts by Western blot and gel shift, respectively. When glucocorticoid levels were low, as during the light period of the circadian cycle, nuclear GRs and GRE binding were almost undetectable. However, the increase in glucocorticoid levels observed during the dark phase of the circadian cycle or after stress induced a massive nuclear translocation of GRs and GRE binding. These effects were corticosterone-dependent because they were suppressed by adrenalectomy and restored by the injection of corticosterone. Furthermore, GR translocation and GRE binding were of higher amplitude or lasted longer in the hippocampus than in the prefrontal cortex. By contrast, extracellular levels of glucocorticoids, measured by microdialysis in freely moving animals, were identical in the two structures. These results suggest that specific intracellular regulations of GR activity contribute to differentiate the effects of glucocorticoids in different regions of the brain. (literal)

Prodotto di

• (SV.P12.003) Individuazione, recupero e conservazione della biodiversità dei lieviti siciliani e loro catalogazione territoriale. (SV.P17.003.001) (Modulo)
• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)

Autore CNR

• FRANCESCO DI BLASI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Differences between brain structures in nuclear translocation and DNA binding of the glucocorticoid receptor during stress and the circadian cycle" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• FRANCESCO DI BLASI (Unità di personale interno)

Prodotto

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• (SV.P12.003) Individuazione, recupero e conservazione della biodiversità dei lieviti siciliani e loro catalogazione territoriale. (SV.P17.003.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• European journal of neuroscience (Rivista)

Insieme di parole chiave di

• Parole chiave di "Differences between brain structures in nuclear translocation and DNA binding of the glucocorticoid receptor during stress and the circadian cycle" (Insieme di parole chiave)