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Identification of enhancer binding protein MBF-1 of the sea urchin modulator a-H2A histone gene (Articolo in rivista)

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Identification of enhancer binding protein MBF-1 of the sea urchin modulator a-H2A histone gene (Articolo in rivista) (literal)

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Alessandro C , Di Simone P, Buscaino A, Anello L, Palla F, Spinelli G. (2002)
Identification of enhancer binding protein MBF-1 of the sea urchin modulator a-H2A histone gene
in Biochemical and biophysical research communications (Print); Academic Press, San Diego (Stati Uniti d'America)
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Alessandro C , Di Simone P, Buscaino A, Anello L, Palla F, Spinelli G. (literal)

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Identification of enhancer binding protein MBF-1 of the sea urchin modulator a-H2A histone gene (literal)

Abstract

The modulator of the sea urchin a-H2A histone gene promoter is the only enhancer identified in the a-histone gene cluster. Binding of a single factor, denoted MBF-1, has previously detected in nuclear extracts from morula and gastrula embryos. Here, we describe the cloning of MBF-1 by screening a cDNA expression library with a tandem array of modulator binding sites. MBF-1 presents no similarity with other DNA binding proteins and contains nine KrEURuppel like Zn fingers. In vitro translated proteins and a factor from nuclear extracts interact with the modulator with identical specificity. In addition, MBF-1 expressed in human cells transactivates a reporter gene driven by an array of modulator sites. The DNA binding domain consists of the Zn fingers plus an adjacent basic region, while sequences in the N-terminal region mediates the transactivation function. MBF-1 is expressed in the unfertilized egg and in early and late developmental stages thus confirming that it is not a stage specific enhancer binding factor and that silencing of the a-H2A gene after hatching is not due to the lack of the transactivator. ? 2002 Elsevier Science (USA). All rights reserved. (literal)

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