Giardia intestinalis escapes oxidative stress by colonizing the small intestine: a molecular hypothesis (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Giardia intestinalis escapes oxidative stress by colonizing the small intestine: a molecular hypothesis (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Alternative label

• Mastronicola D; Giuffrè A; Testa F; Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P. (2011)
  Giardia intestinalis escapes oxidative stress by colonizing the small intestine: a molecular hypothesis
  in IUBMB life (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Mastronicola D; Giuffrè A; Testa F; Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P. (literal)

Pagina inizio

• 21 (literal)

Pagina fine

• 25 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 63 (literal)

Rivista

• IUBMB life (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Department of Biochemical Sciences, CNR Institute of Molecular Biology and Pathology and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza University of Rome, Rome, Italy; Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy; Clinical Chemistry and Microbiology Laboratory, Istituto Nazionale per le Malattie Infettive I.R.C.C.S. ''Lazzaro Spallanzani,'' Rome, Italy; Center for Biotechnology Development and Biodiversity Research, University of Sassari, Sassari, Italy. (literal)

Titolo

• Giardia intestinalis escapes oxidative stress by colonizing the small intestine: a molecular hypothesis (literal)

Abstract

• Giardia intestinalis is the microaerophilic protozoon causing giardiasis, a common infectious intestinal disease. Giardia possesses an O2-scavenging activity likely essential for survival in the host. We report that Giardia trophozoites express the O2-detoxifying flavodiiron protein (FDP), detected by immunoblotting, and are able to reduce O2 to H2O rapidly (?3 lM O2 3 min 3 106 cells at 37 8C) and with high affinity (C50 5 3.4 6 0.7 lM O2). Following a short-term (minutes) exposure to H2O2 ? 100 lM, the O2 consumption by the parasites is irreversibly impaired, and the FDP undergoes a degradation, prevented by the proteasome-inhibitor MG132. Instead, H2O2 does not cause degradation or inactivation of the isolated FDP. On the basis of the elevated susceptibility of Giardia to oxidative stress, we hypothesize that the parasite preferentially colonizes the small intestine since, compared with colon, it is characterized by a greater capacity for redox buffering and a lower propensity to oxidative stress. (literal)

Prodotto di

• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)
• Institute of molecular biology and pathology (IBPM) (Istituto)

Autore CNR

• ALESSANDRO GIUFFRE' (Unità di personale interno)
• DANIELA MASTRONICOLA (Unità di personale esterno)

Incoming links:

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR di

• ALESSANDRO GIUFFRE' (Unità di personale interno)
• DANIELA MASTRONICOLA (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• IUBMB life (Rivista)