Lsm Proteins Promote Regeneration of Pre-mRNA Splicing Activity (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Lsm Proteins Promote Regeneration of Pre-mRNA Splicing Activity (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Alternative label

• Loredana Verdone,1,4; Silvia Galardi,2,4; David Page,3; and Jean D. Beggs* (2004)
  Lsm Proteins Promote Regeneration of Pre-mRNA Splicing Activity
  in Current biology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Loredana Verdone,1,4; Silvia Galardi,2,4; David Page,3; and Jean D. Beggs* (literal)

Pagina inizio

• 1487 (literal)

Pagina fine

• 1491 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 14 (literal)

Rivista

• Current biology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• *Correspondence: jbeggs@ed.ac.uk 1Present address: Department of Genetics and Molecular Biology, University of Rome \"La Sapienza,\" 00185 Rome, Italy. 2Present address: Department of Experimental Medicine, University of Rome \"Tor Vergata,\" 00133 Rome, Italy. 3 Present address: ExpressOn BioSystems Ltd, The Logan Building, Roslin Biocentre, Edinburgh, EH25 9TT, United Kingdom. 4These authors contributed equally to this work. (literal)

Titolo

• Lsm Proteins Promote Regeneration of Pre-mRNA Splicing Activity (literal)

Abstract

• Lsm proteins are ubiquitous, multifunctional proteins that affect the processing of most RNAs in eukaryotic cells, but their function is unknown. A complex of seven Lsm proteins, Lsm2-8, associates with the U6 small nuclear RNA (snRNA) that is a component of spliceosome complexes in which pre-mRNA splicing occurs. Spliceosomes contain five snRNAs, U1, U2, U4, U5, and U6, that are packaged as ribonucleoprotein particles (snRNPs). U4 and U6 snRNAs contain extensive sequence complementarity and interact to form U4/U6 di-snRNPs. U4/U6 di-snRNPs associate with U5 snRNPs to form U4/U6.U5 tri-snRNPs prior to spliceosome assembly. Within spliceosomes, disruption of base-paired U4/U6 heterodimer allows U6 snRNA to form part of the catalytic center. Following completion of the splicing reaction, snRNPs must be recycled for subsequent rounds of splicing, although little is known about this process. Here we present evidence that regeneration of splicing activity in vitro is dependent on Lsm proteins. RNP reconstitution experiments with exogenous U6 RNA show that Lsm proteins promote the formation of U6-containing complexes and suggest that Lsm proteins have a chaperone-like function, supporting the assembly or remodeling of RNP complexes involved in splicing. Such a function could explain the involvement of Lsm proteins in a wide variety of RNA processing pathways. (literal)

Prodotto di

• Struttura e funzione di Acidi nucleici e Cromatina. Epigenetica (SV.P14.009.001) (Modulo)
• Institute of molecular biology and pathology (IBPM) (Istituto)

Autore CNR

• LOREDANA VERDONE (Unità di personale interno)

Incoming links:

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Struttura e funzione di Acidi nucleici e Cromatina. Epigenetica (SV.P14.009.001) (Modulo)

Autore CNR di

• LOREDANA VERDONE (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Current biology (Rivista)