Detection of 16SrI-B subgroup phytoplasmas associated with virescence, phyllody and severe growth abnormalities in ranunculus in Campania (Southern Italy). (Abstract/Poster in atti di convegno)

Type
Label
  • Detection of 16SrI-B subgroup phytoplasmas associated with virescence, phyllody and severe growth abnormalities in ranunculus in Campania (Southern Italy). (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Alternative label
  • Parrella G., Botti S., Paltrinieri S., Matteucig G., BertaccinI A. (2006)
    Detection of 16SrI-B subgroup phytoplasmas associated with virescence, phyllody and severe growth abnormalities in ranunculus in Campania (Southern Italy).
    in 13th Congresso Nazionale della Società Italiana di Patologia Vegetale,, Foggia
    (literal)
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  • Parrella G., Botti S., Paltrinieri S., Matteucig G., BertaccinI A. (literal)
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  • Journal of Plant Pathology (2006), 88 (3, Supplement), S53. 13th Congresso Nazionale della Società Italiana di Patologia Vegetale, 12-15 settembre, Foggia. (literal)
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  • In the late 2005 ranunculus hybrids with phytoplasma-like symptoms were observed in two protected cultivations in Campania (Southern Italy). Before flowering, symptoms included severe growth abnormalities, shoot proliferation and decline; later on virescence and phyllody of the flowers was observed. PCR using phytoplasma-specific primer pairs confirmed the presence of phytoplasma DNA in the infected plants. Sequence analysis of the 16S rDNA amplicon obtained with P1/P7 primer pair (ca. 1800 bp), revealed high homology (99%) with phytoplasma DNAs in the database. Based on phylogenetic and RFLP analysis of this amplicon carried out with TruI, Tsp509I, SspI, RsaI and HhaI the ranunculus phytoplasma was identified as an isolate of ‘Candidatus Phytoplasma asteris’ (aster yellows), subgroup 16SrIB. Three leafhopper species (Homoptera, Cicadellidae) captured with yellow sticky traps were associated with ranunculus cultivations: Empoasca decipiens (Paoli), Eupteryx zelleri (Kirschbaum) and Zygina sp. Batches of ten individuals per species were tested for the presence of phytoplasmas by nested-PCR/RFLP analyses using primers P1/P7 followed by primers F1/B6. Only E. decipiens samples were positive and RFLP analysis of the F1/B6 PCR products (ca. 1700 bp), revealed the association to subgroup 16SrI-B phytoplasma in two batches, whereas in another batch, a restriction profile referable to the 16SrII group was obtained. Although E. decipiens already been reported to be associated to phytoplasmas, its the role as vector must be proven. This is the first report of 16SrI-B subgroups phytoplasmas in ranunculus in Italy. (literal)
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  • - Parrella G., CNR, Ist. Protezione Piante - Sez. di Portici (NA) (literal)
Titolo
  • Detection of 16SrI-B subgroup phytoplasmas associated with virescence, phyllody and severe growth abnormalities in ranunculus in Campania (Southern Italy). (literal)
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