Molecular survey on tomato yellow leaf curl disease and its vector in Italy. (Abstract in rivista)

Type
Label
  • Molecular survey on tomato yellow leaf curl disease and its vector in Italy. (Abstract in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Alternative label
  • Parrella G., Scassillo L., Crescenzi A., Nappo A.G. (2005)
    Molecular survey on tomato yellow leaf curl disease and its vector in Italy.
    in XII Congresso Nazionale SIPaV, Villa S. Giovanni (RC), 2005
    (literal)
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  • Parrella G., Scassillo L., Crescenzi A., Nappo A.G. (literal)
Convegno
  • XII Congresso Nazionale SIPaV (literal)
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  • Villa S. Giovanni (RC) (literal)
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  • Journal of Plant Pathology, 87 (4, Special issue), 302. XII Congresso Nazionale SIPaV, 29 settembre-1 ottobre, S. Trada di Cannitello, Villa S. Giovanni (RC). (literal)
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  • A molecular survey of viruses responsible for tomato yellow leaf curl disease (TYLCD) and their associated vector Bemisia tabaci Genn., was conducted during 2004-2005 in four regions of southern Italy: i.e. Sardinia (two locations), Sicily (one location), Calabria (three locations) and Campania (two locations). A total of 62 tomato samples were checked for virus infection and for the presence of the vector. In a preliminary screening plants were tested for the presence of Tomato yellow leaf curl Sardinia virus (TYLCSV) and Tomato yellow leaf curl virus (TYLCV) by PCRRFLP of a 680 bp fragment comprising the coat protein gene. Results clearly showed that in all four regions both viruses are widespread and present singly or in mixture in single plants. The identity of the two viruses was confirmed by total or partial sequencing of field isolates. In particular, the majority of TYLCV isolates found in Italy showed high similarity with TYLCV mild strain (TYLCV-Mld), whereas other isolates shared the highest similarity with an Egyptian isolate (TYLCV-EG). TYLCSV isolates showed a high level of identity with TYLCSV or TYLCSV from Sicily (TYLCSV-Sic). Concerning B. tabaci populations associated with TYLCD epidemics, the molecular characterization of COI gene (citocrome oxidase I) showed that the Q biotype was the most prevalent. This may be a consequence of the large use of insecticides against which Q biotype populations easily develop resistance, as observed in some countries of the Mediterranean basin. (literal)
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  • - Nappo A.G., Parrella G., CNR, Ist. Protezione Piante - Sez. di Portici (NA) (literal)
Titolo
  • Molecular survey on tomato yellow leaf curl disease and its vector in Italy. (literal)
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