Characterization of a Potato virus Y isolate from Capsicum chinense cv. Red Habanero. (Abstract in rivista)

Type
Label
  • Characterization of a Potato virus Y isolate from Capsicum chinense cv. Red Habanero. (Abstract in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Alternative label
  • Comes S., Fanigliulo A., Pacella R., Parrella G., Crescenzi A. (2005)
    Characterization of a Potato virus Y isolate from Capsicum chinense cv. Red Habanero.
    in XII Congresso Nazionale SIPaV, Villa S. Giovanni (RC), 2005
    (literal)
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  • Comes S., Fanigliulo A., Pacella R., Parrella G., Crescenzi A. (literal)
Convegno
  • XII Congresso Nazionale SIPaV (literal)
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  • Villa S. Giovanni (RC) (literal)
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  • Journal of Plant Pathology, 87 (4, Special issue), 292. XII Congresso Nazionale SIPaV, 29 settembre-1 ottobre, S. Trada di Cannitello, Villa S. Giovanni (RC). (literal)
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  • Plants of chili pepper, Capsicum chinense Jacquin cv Red Habanero, with leaf mosaic, necrotic rings on the fruits, and stem necrosis were observed in June 2003 in a private garden of Naples (southern Italy). Preliminary serological characterisation showed that these symptoms were associated with the presence of Potato virus Y (PVY). This virus was isolated in Nicotiana tabacum cv Xanthi NC and characterised biologically and molecularly in its coat protein (CP) gene. Symptoms produced in indicators were generally consistent with those described for PVY, i.e leaf deformation and veinal necrosis in N. occidentalis, mosaic in N. tabacun cv Xanthi and Capsicum annuum, mosaic and epinasty in Lycopersicon esculentum. By contrast, the virus induced local symptoms of chlorotic mottling in Chenopodium spp.. When the viral isolate was exposed to monoclonal antibodies (Mabs) specific to PVYN, PVYC and PVYO, it reacted only with PVYC Mabs. RT-PCR was done on viral RNA extracted from purified virions using a couple of primers specifically designed on the CP coding region of PVYNP (non potato) isolates. PCR product was cloned in a pCRII-TOPO vector using TOPO-TA cloning kit (Invitrogen) and sequenced. Both similarity and phylogenetic analysis made on the 801 bp long coat CP gene sequence showed that the isolate under study is more closely related to other PVY non potato isolates than to those of the PVYO and PVYN group. The highest percentage of similarity was detected with PVY isolates from Solanum nigrum (PVY-SON41), Capsicum annuum (PVY-NNP) and Lycopersicon esculentum (PVY-LYE84.2). (literal)
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  • - Parrella G., CNR, Ist. Protezione Piante - Sez. di Portici (NA) (literal)
Titolo
  • Characterization of a Potato virus Y isolate from Capsicum chinense cv. Red Habanero. (literal)
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