Drosophila Dgt6 protein interacts with Ndc80, Msps/XMAP215 and g-tubulin to promote kinetochore-driven MT formation (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Drosophila Dgt6 protein interacts with Ndc80, Msps/XMAP215 and g-tubulin to promote kinetochore-driven MT formation (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Alternative label

• E. Bucciarelli; C. Pellacani; V. Naim; A. Palena; M. Gatti; M.P. Somma (2009)
  Drosophila Dgt6 protein interacts with Ndc80, Msps/XMAP215 and g-tubulin to promote kinetochore-driven MT formation
  in Current biology; Cell Press, Cambridge (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• E. Bucciarelli; C. Pellacani; V. Naim; A. Palena; M. Gatti; M.P. Somma (literal)

Pagina inizio

• 1839 (literal)

Pagina fine

• 1845 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 19 (literal)

Rivista

• Current biology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 21 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto Pasteur-Fondazione Cenci Bolognetti and Istituto di Biologia e Patologia Molecolari del Consiglio Nazionale delle Ricerche (CNR), Dipartimento di Genetica e Biologia Molecolare, Università di Roma \"La Sapienza,\" P.A. Moro 5, 00185 Roma, Ita (literal)

Titolo

• Drosophila Dgt6 protein interacts with Ndc80, Msps/XMAP215 and g-tubulin to promote kinetochore-driven MT formation (literal)

Abstract

• In centrosome-containing cells, spindle assembly relies on microtubules (MTs) nucleated from both centrosomes and chromosomes. Recent work has suggested that additional spindle MTs can be nucleated by gamma-tubulin ring complexes (gamma-TuRCs) that associate laterally with preexisting spindle MTs, leading to spindle amplification. It has been proposed that in Drosophila S2 cells, gamma-TuRCs are anchored to the spindle MTs by augmin, a multiprotein complex that contains at least eight subunits. Here we show that the Dgt6 component of augmin is primarily required for kinetochore fiber (k-fiber) formation. An analysis of MT regrowth after cold exposure showed that formation of kinetochore-driven k-fibers is severely impaired in Dgt6-depleted cells. In control cells, these fibers are enriched in Dgt6, gamma-tubulin, and Msps/XMAP215. Consistent with these results, Dgt6 coprecipitates with Msps, D-TACC, gamma-tubulin, Ndc80, and Nuf2. However, RNA interference (RNAi)-mediated inhibition of gamma-tubulin, Msps/XMAP215, or Ndc80/Hec1 reduced but did not abolish k-fiber regrowth. These results indicate that Dgt6 plays a pivotal role in kinetochore-driven k-fiber formation, mediating nucleation and/or initial stabilization of chromosome-induced MTs. We propose that Dgt6 binds and stabilizes nascent chromatin-induced MTs, facilitating their interaction with the Ndc80-Nuf2 complex. Dgt6 may also promote elongation of kinetochore-driven k-fibers through its interaction with gamma-tubulin and Msps. (literal)

Editore

• Cell Press (Editore)

Prodotto di

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Meccanismi molecolari del ciclo cellulare e della mitosi (SV.P15.011.001) (Modulo)

Autore CNR

• ANTONELLA PALENA (Unità di personale interno)
• MAURIZIO GATTI (Unità di personale esterno)
• MARIA PATRIZIA SOMMA (Persona)
• ELISABETTA BUCCIARELLI (Unità di personale esterno)

Incoming links:

Autore CNR di

• MAURIZIO GATTI (Unità di personale esterno)
• ANTONELLA PALENA (Unità di personale interno)
• MARIA PATRIZIA SOMMA (Persona)
• ELISABETTA BUCCIARELLI (Unità di personale esterno)

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Meccanismi molecolari del ciclo cellulare e della mitosi (SV.P15.011.001) (Modulo)

Editore di

• Cell Press (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Current biology (Rivista)