The role of antioxidant enzymes in the virulence of root-knot nematodes on resistant tomato. (Abstract/Poster in atti di convegno)

Type
Label
  • The role of antioxidant enzymes in the virulence of root-knot nematodes on resistant tomato. (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Alternative label
  • Molinari S., Rosso L., Ornat Longaron C. (2005)
    The role of antioxidant enzymes in the virulence of root-knot nematodes on resistant tomato.
    in XXXVII Onta Annual Meeting, Viña del Mar, Chile
    (literal)
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  • Molinari S., Rosso L., Ornat Longaron C. (literal)
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  • Nematropica, 35(2), 88. Proceedings of the XXXVII Onta Annual Meeting, October 2005, Viña del Mar, Chile. (literal)
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  • Resistance to the major root-knot nematodes (RKN, Meloidogyne spp.) is conferred by the dominant R-gene Mi in tomato. Several resistance-breaking Meloidogyne populations have already been isolated from the field in different countries. Virulent isolates can also be selected from wild populations by repeated inoculations on resistant tomato. In this study, a virulent isolate of Meloidogyne incognita (SM1and avr1) coming from the same starting population have been compared as it concerns their antioxidant enzyme activities. Expression of Mn-SOD in SM1 and avr1 J2 was detected by RT-PCR analysis. The same amount of cDNA from both isolates was used as template and PCR primers were constructed according to an EST cDNA sequence (BI773441) sharing high similarity with Mn-SOD (sod-2) from Caenorhabdtis elegans. The single amplified DNA fragment obtained (297 bp) was much more evident in the virulent isolate SM1 than in the avirulent counterpart avr1. Moreover, total and H2O2-resistant SOD activity was markedly higher in J2 extracts from SM1. Generally, all the antioxidant activities tested were higher in virulent isolates. Hence, this study indicates that enhanced antioxidant activities are most probably related to the ability of virulent J2 to develop on Mi-bearing tomato plants. (literal)
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  • - Molinari S., Rosso L., CNR, Ist. Protezione Piante - Sez. di Bari (literal)
Titolo
  • The role of antioxidant enzymes in the virulence of root-knot nematodes on resistant tomato. (literal)
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