Cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from Thalictrum flavum. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from Thalictrum flavum. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1107/S1744309108005678 (literal)

Alternative label

• Pasquo A, Bonamore A, Franceschini S, Macone A, Boffi A, Ilari A. (2008)
  Cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from Thalictrum flavum.
  in Acta crystallographica. Section F
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Pasquo A, Bonamore A, Franceschini S, Macone A, Boffi A, Ilari A. (literal)

Pagina inizio

• 281 (literal)

Pagina fine

• 283 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 64 (literal)

Rivista

• Acta crystallographica. Section F. Structural biology and crystallization communications (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Univ Roma La Sapienza, CNR, IBPM, I-00719 Rome, Italy 2. Univ Roma La Sapienza, Dept Biochem Sci, I-00719 Rome, Italy 3. ENEA, Casaccia Res Ctr, Dipartimento BIOTEC, Sez Genet & Genom Vegetale, I-00100 Rome, Italy (literal)

Titolo

• Cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from Thalictrum flavum. (literal)

Abstract

• Norcoclaurine synthase ( NCS) catalyzes the condensation of 3,4-dihydroxy-phenylethylamine ( dopamine) and 4-hydroxyphenylacetaldehyde (4-HPAA) as the first committed step in the biosynthesis of benzylisoquinoline alkaloids in plants. The protein was cloned, expressed and purified. Crystals were obtained at 294 K by the hanging-drop vapour-diffusion method using ammonium sulfate and sodium chloride as precipitant agents and diffract to better than 3.0 angstrom resolution using a synchrotron-radiation source. The crystals belong to the trigonal space group P3(1)21, with unit-cell parameters a = b = 86.31, c = 118.36 angstrom. A selenomethionine derivative was overexpressed, purified and crystallized in the same space group. A complete MAD data set was collected at 2.7 angstrom resolution. The model is under construction. (literal)

Prodotto di

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR

• ANDREA ILARI (Unità di personale interno)
• STEFANO FRANCESCHINI (Unità di personale esterno)
• ALBERTO BOFFI (Unità di personale esterno)

Incoming links:

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR di

• ANDREA ILARI (Unità di personale interno)
• ALBERTO BOFFI (Unità di personale esterno)
• STEFANO FRANCESCHINI (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Acta crystallographica. Section F. Structural biology and crystallization communications (Rivista)