Trypanothione reductase from Leishmania infantum: cloning, expression, purification, crystallization and preliminary X-ray data analysis. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Trypanothione reductase from Leishmania infantum: cloning, expression, purification, crystallization and preliminary X-ray data analysis. (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Alternative label

• Baiocco P; Franceschini S; Ilari A; Colotti G. (2009)
  Trypanothione reductase from Leishmania infantum: cloning, expression, purification, crystallization and preliminary X-ray data analysis.
  in Protein and peptide letters (Print); Bentham Science Publishers Ltd., Sharjah (Emirati Arabi Uniti)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Baiocco P; Franceschini S; Ilari A; Colotti G. (literal)

Pagina inizio

• 196 (literal)

Pagina fine

• 200 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.benthamdirect.org/pages/content.php?PPL/2009/00000016/00000002/0014E.SGM (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 16 (literal)

Rivista

• Protein and peptide letters (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 2 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Univ Roma La Sapienza, Dept Biochem Sci, IBPM, CNR, I-00185 Rome, Italy (literal)

Titolo

• Trypanothione reductase from Leishmania infantum: cloning, expression, purification, crystallization and preliminary X-ray data analysis. (literal)

Abstract

• The most promising targets for Leishmania-specific drug design are two key enzymes involved in the unique thiol-based metabolism, common to all parasites of the Trypanosomatidae family: trypanothione synthetase (TryS) and trypanothione reductase (TR). Recently, new inhibitors of TR have been identified such as polyamines and tricyclic compounds. The knowledge of the three-dimensional structure of Leishmania TR will shed light on the mechanism of interaction of these inhibitors with TR and will be the starting point to design novel lead candidates to facilitate the development of new effective and affordable drugs. Trypanothione reductase from Leishmania infantum has been cloned, expressed in E. coli and purified. Crystals were obtained at 294 K by the hanging drop vapour diffusion method using ammonium sulfate as precipitant agent and diffract to better than 2.95 angstrom resolution using a synchrotron radiation source. The crystals exhibit an unusually high solvent content of 74 %, belong to the tetragonal space group P41 with units cell parameters a=b=103.45 angstrom, c=192.62 angstrom and two molecules in the asymmetric unit. The protein X-ray structure has been solved by Molecular Replacement and the model is under construction. (literal)

Editore

• Bentham Science Publishers Ltd. (Editore)

Prodotto di

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR

• ANDREA ILARI (Unità di personale interno)
• STEFANO FRANCESCHINI (Unità di personale esterno)
• GIANNI COLOTTI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Trypanothione reductase from Leishmania infantum: cloning, expression, purification, crystallization and preliminary X-ray data analysis." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR di

• GIANNI COLOTTI (Unità di personale interno)
• ANDREA ILARI (Unità di personale interno)
• STEFANO FRANCESCHINI (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Protein and peptide letters (Rivista)

Editore di

• Bentham Science Publishers Ltd. (Editore)

Insieme di parole chiave di

• Keywords of "Trypanothione reductase from Leishmania infantum: cloning, expression, purification, crystallization and preliminary X-ray data analysis." (Insieme di parole chiave)