The virF promoter in Shigella: more than just a curved DNA stretch (Articolo in rivista)

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  • The virF promoter in Shigella: more than just a curved DNA stretch (Articolo in rivista) (literal)
Anno
  • 2004-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1046/j.1365-2958.2003.03848.x (literal)
Alternative label
  • Gianni Prosseda; Maurizio Falconi; Mara Giangrossi; Claudio O. Gualerzi; Gioacchino Micheli; Bianca Colonna (2004)
    The virF promoter in Shigella: more than just a curved DNA stretch
    in Molecular microbiology (Print); Blackwell Publishing, Oxford (Regno Unito)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Gianni Prosseda; Maurizio Falconi; Mara Giangrossi; Claudio O. Gualerzi; Gioacchino Micheli; Bianca Colonna (literal)
Pagina inizio
  • 523 (literal)
Pagina fine
  • 537 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2003.03848.x/abstract;jsessionid=F86A73F678A1978B71912FE64F6622F0.d01t03 (literal)
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  • 51 (literal)
Rivista
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  • 2 (literal)
Note
  • Google Scholar (literal)
  • Scopu (literal)
  • ISI Web of Science (WOS) (literal)
  • PubMe (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • * Dip. Biologia Cellulare e dello Sviluppo, University La Sapienza, 00185 Roma, Italy. * Dip. Biologia MCA, University Camerino, 62032 Camerino MC, Italy. * Dip. Biologia MCA, University Camerino, 62032 Camerino MC, Italy. * Dip. Biologia MCA, University Camerino, 62032 Camerino MC, Italy. * Istituto di Biologia e Patologia Molecolari CNR, Sez. Acidi Nucleici, University La Sapienza, 00185 Roma, Italy. * Dip. Biologia Cellulare e dello Sviluppo, University La Sapienza, 00185 Roma, Italy. (literal)
Titolo
  • The virF promoter in Shigella: more than just a curved DNA stretch (literal)
Abstract
  • In the human enteropathogen Shigella transcription of virF, the primary regulator of the invasion functions, is strictly temperature-dependent and is antagonistically mediated by H-NS and FIS, which bind to specific sites on the virF promoter. Here we report on the relevance of DNA geometry to the themoregulation of virF and demonstrate that the virF promoter hosts a major DNA bend halfway between two H-NS sites. The bent region has been mutagenized in vitro to mimic temperature-induced changes of DNA curvature. Functional analysis of curvature mutants and of promoter constructs in which the two H-NS sites are phased-out by a half-helix turn reveals that modifying the spatial relationships between these sites severely affects the interaction of H-NS with the virF promoter, as well as its in vivo and in vitro temperature-dependent activity. The role of promoter curvature as thermosensor is also compatible with the present observation that, with increasing temperature, the virF bending centre moves downstream at a rate having its maximum around the transition temperature, abruptly unmasking a binding site for the transcriptional activator FIS. (literal)
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