http://www.cnr.it/ontology/cnr/individuo/prodotto/ID11609
Identification of Drosophila mitotic genes by combining co-expression analysis and RNA interference. (Articolo in rivista)
- Type
- Label
- Identification of Drosophila mitotic genes by combining co-expression analysis and RNA interference. (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- M.P. Somma; F. Ceprani; E. Bucciarelli; V. Naim; V. De Arcangelis; R. Piergentili; A. Palena; L. Ciapponi; M.G. Giansanti; C. Pellacani; R. Petrucci; G. Cenci; F. Vernì; B. Fasulo; M.L. Goldberg; F. Di Cunto; M. Gatti (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- CNR, Università di Roma \"Sapienza\", Università di Torino, Cornell University Ithaca USA (literal)
- Titolo
- Identification of Drosophila mitotic genes by combining co-expression analysis and RNA interference. (literal)
- Abstract
- Abstract
RNAi screens have, to date, identified many genes required for mitotic divisions of Drosophila tissue culture cells.
However, the inventory of such genes remains incomplete. We have combined the powers of bioinformatics and RNAi
technology to detect novel mitotic genes. We found that Drosophila genes involved in mitosis tend to be transcriptionally
co-expressed. We thus constructed a co-expression-based list of 1,000 genes that are highly enriched in mitotic functions,
and we performed RNAi for each of these genes. By limiting the number of genes to be examined, we were able to
perform a very detailed phenotypic analysis of RNAi cells. We examined dsRNA-treated cells for possible abnormalities in
both chromosome structure and spindle organization. This analysis allowed the identification of 142 mitotic genes, which
were subdivided into 18 phenoclusters. Seventy of these genes have not previously been associated with mitotic defects;
30 of them are required for spindle assembly and/or chromosome segregation, and 40 are required to prevent
spontaneous chromosome breakage. We note that the latter type of genes has never been detected in previous RNAi
screens in any system. Finally, we found that RNAi against genes encoding kinetochore components or highly conserved
splicing factors results in identical defects in chromosome segregation, highlighting an unanticipated role of splicing
factors in centromere function. These findings indicate that our co-expression-based method for the detection of mitotic
functions works remarkably well. We can foresee that elaboration of co-expression lists using genes in the same
phenocluster will provide many candidate genes for small-scale RNAi screens aimed at completing the inventory of
mitotic proteins.
Citation: (literal)
- Prodotto di
- Autore CNR
Incoming links:
- Autore CNR di
- Prodotto
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi