Drosophila Spd-2 is an essential centriole component required for PCM recruitment and astral microtubule nucleation. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Drosophila Spd-2 is an essential centriole component required for PCM recruitment and astral microtubule nucleation. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.cub.2008.01.058 (literal)

Alternative label

• Giansanti MG.; Bucciarelli E.; Bonaccorsi S.; Gatti M (2008)
  Drosophila Spd-2 is an essential centriole component required for PCM recruitment and astral microtubule nucleation.
  in Current biology; Cell Press, Cambridge (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Giansanti MG.; Bucciarelli E.; Bonaccorsi S.; Gatti M (literal)

Pagina inizio

• 303 (literal)

Pagina fine

• 309 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 18 (literal)

Rivista

• Current biology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 4 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Univ Roma La Sapienza, Dipartimento Genet & Biol Mol, Ist Pasteur Fdn Cenci Bolognetti, I-00185 Rome, Italy 2. Univ Roma La Sapienza, Dipartimento Genet & Biol Mol, Ist Biol & Patol Mol, CNR, I-00185 Rome, Italy (literal)

Titolo

• Drosophila Spd-2 is an essential centriole component required for PCM recruitment and astral microtubule nucleation. (literal)

Abstract

• SPD-2 is a C. elegans centriolar protein required for both centriole duplication and pericentriolar material (PCM) recruitment [1-4]. SPD-2 is conserved in Drosophila (DSpd-2) and is a component of the fly centriole [5-7]. The analysis of a P element-induced hypomorphic mutation has shown that DSpd-2 is primarily required for PCM recruitment at the sperm centriole but is dispensable for both centriole duplication and aster formation [5]. Here we show that null mutations carrying early stop codons in the DSpd-2 coding sequence suppress astral microtubule (MT) nucleation in both neuroblasts (NBs) and spermatocytes. These mutations also disrupt proper Miranda localization in dividing NBs, as previously observed in mutants lacking astral MTs [8-10]. Spermatocyte analysis revealed that DSpd-2 is enriched at both the centrioles and the PCM and is required for the maintenance of cohesion between the two centrioles but not for centriole duplication. We found that DSpd-2 localization at the centrosome requires the wild-type activity of Asl but is independent of the function of D-PLP, Cnn, gamma-tubulin, DGrip91, and D-TACC. Conversely, DSpd-2 mutants displayed normal centrosomal accumulations of Asl and D-PLP, strongly reduced amounts of Cnn, gamma-tubulin, and DGrip91, and diffuse localization of D-TACC. These results indicate that DSpd-2 functions in a very early step of the PCM recruitment pathway. (literal)

Editore

• Cell Press (Editore)

Prodotto di

• Meccanismi molecolari del ciclo cellulare e della mitosi (SV.P15.011.001) (Modulo)
• Institute of molecular biology and pathology (IBPM) (Istituto)

Autore CNR

• ELISABETTA BUCCIARELLI (Unità di personale esterno)
• MAURIZIO GATTI (Unità di personale esterno)
• MARIA GRAZIA GIANSANTI (Persona)

Insieme di parole chiave

• Keywords of "Drosophila Spd-2 is an essential centriole component required for PCM recruitment and astral microtubule nucleation." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MAURIZIO GATTI (Unità di personale esterno)
• MARIA GRAZIA GIANSANTI (Persona)
• ELISABETTA BUCCIARELLI (Unità di personale esterno)

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Meccanismi molecolari del ciclo cellulare e della mitosi (SV.P15.011.001) (Modulo)

Editore di

• Cell Press (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Current biology (Rivista)

Insieme di parole chiave di

• Keywords of "Drosophila Spd-2 is an essential centriole component required for PCM recruitment and astral microtubule nucleation." (Insieme di parole chiave)