Influence of caspase activity on micronuclei detection: a possible role for caspase-3 inmicronucleation. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Influence of caspase activity on micronuclei detection: a possible role for caspase-3 inmicronucleation. (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Alternative label

• Decordier I, Cundari E, Kirsch-Volders M. (2005)
  Influence of caspase activity on micronuclei detection: a possible role for caspase-3 inmicronucleation.
  in Mutagenesis
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Decordier I, Cundari E, Kirsch-Volders M. (literal)

Pagina inizio

• 173 (literal)

Pagina fine

• 179 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 20 (literal)

Rivista

• Mutagenesis (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Vrije Universiteit Brussel, Laboratorium voor Cellulaire Genetica, Pleinlaan 2, B-1050 Brussel, Belgium and 1Istituto di biologia e patologia molecolari, C.N.R., Via degli Apuli 4, I-00185 Roma, Italy (literal)

Titolo

• Influence of caspase activity on micronuclei detection: a possible role for caspase-3 inmicronucleation. (literal)

Abstract

• Aneugenic and clastogenic agents are good inducers of both micronuclei and apoptosis. In its turn, apoptosis may modify the threshold values for the induction of micronuclei. This is of major concern for accurate assessment of hazard related to exposure to mutagens. In the present work we studied the influence of caspases, the key regulators of the apoptotic process, on the induction of micronuclei in the cytokinesis block micronucleus assay. For this, we applied a combined approach in which both human peripheral blood mononucleated cells (PBMC) and the paired human breast carcinoma cell lines MCF-7, which is caspase-3 deficient, and the caspase-3 transfected MCF-7 (MCF-7casp-3) were used to study the influence of caspase activity on micronuclei. When nocodazole induced apoptosis was inhibited by the use of inhibitors of the two main apical caspases-8 and -9 in PBMC, the frequencies of micronucleated binucleates (MNCB) increased with inhibition of these caspases confirming that apoptosis can eliminate micronucleated cells. On the contrary when caspase-3 was inhibited, the frequencies of MNCB was lower, suggesting a role of caspase-3, also in micronuclei formation. To verify this hypothesis, we compared the induction of apoptosis and micronuclei by the aneugen nocodazole, the clastogen methyl methane sulfonate (MMS) and the non-mutagenic apoptogen staurosporin in MCF-7 and MCF-7casp-3 cells. The results showed that when caspase-3 activity was impaired, in the parental MCF-7 cell line or in the MCF-7casp-3 cells in the presence of the caspase-3 inhibitor, the frequencies of nocodazole or MMS induced micronuclei decreased. These results suggest that caspase- 3, besides its function as an effector caspase in the apoptotic pathway, is also involved in the formation of micronuclei. (literal)

Prodotto di

• Meccanismi molecolari del ciclo cellulare e della mitosi (SV.P15.011.001) (Modulo)
• Institute of molecular biology and pathology (IBPM) (Istituto)

Autore CNR

• ENRICO CUNDARI (Persona)

Incoming links:

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Meccanismi molecolari del ciclo cellulare e della mitosi (SV.P15.011.001) (Modulo)

Autore CNR di

• ENRICO CUNDARI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Mutagenesis (Rivista)