Feo, the Drosophila homolog of PRC1, is required for central spindle formation and cytokinesis (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Feo, the Drosophila homolog of PRC1, is required for central spindle formation and cytokinesis (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.cub.2004.08.054 (literal)

Alternative label

• F. Vernì; M.P. Somma; K.C. Gunsalus; S. Bonaccorsi; G. Belloni; M.L. Goldberg; M. Gatti (2004)
  Feo, the Drosophila homolog of PRC1, is required for central spindle formation and cytokinesis
  in Current biology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• F. Vernì; M.P. Somma; K.C. Gunsalus; S. Bonaccorsi; G. Belloni; M.L. Goldberg; M. Gatti (literal)

Pagina inizio

• 1569 (literal)

Pagina fine

• 1575 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 14 (literal)

Rivista

• Current biology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Università di Roma \"Sapienza\", CNR, Cornell University Ithaca USA (literal)

Titolo

• Feo, the Drosophila homolog of PRC1, is required for central spindle formation and cytokinesis (literal)

Abstract

• We performed a functional analysis of fascetto (feo), a Drosophila gene that encodes a protein homologous to the Ase1p/PRC1/MAP65 conserved family of microtubule-associated proteins (MAPS) [1-5]. These MAPS are enriched at the spindle midzone in yeast and mammals and at the fragmoplast in plants, and are essential for the organization and function of these microtubule arrays [1-5]. Here we show that the Feo protein is specifically enriched at the central-spindle midzone and that its depletion either by mutation or by RNAi results in aberrant central spindles. In Feo-depleted cells, late anaphases showed normal overlap of the antiparallel MTs at the cell equator, but telophases displayed thin MT bundles of uniform width instead of robust hourglass-shaped central spindles. These thin central spindles exhibited diffuse localizations of both the Pav and Asp proteins, suggesting that these spindles comprise improperly oriented MTs. Feo-depleted cells also displayed defects in the contractile apparatus that correlated with those in the central spindle; late anaphase cells formed regular contractile structures, but these structures did not constrict during telophase, leading to failures in cytokinesis. The phenotype of Feo-depleted telophases suggests that Feo interacts with the plus ends of central spindle MTs so as to maintain their precise interdigitation during anaphase-telophase MT elongation and antiparallel sliding. (literal)

Prodotto di

• Institute of molecular biology and pathology (IBPM) (Istituto)

Autore CNR

• MAURIZIO GATTI (Unità di personale esterno)
• MARIA PATRIZIA SOMMA (Persona)
• SILVIA BONACCORSI (Unità di personale interno)

Incoming links:

Autore CNR di

• MAURIZIO GATTI (Unità di personale esterno)
• MARIA PATRIZIA SOMMA (Persona)
• SILVIA BONACCORSI (Unità di personale interno)

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Current biology (Rivista)