http://www.cnr.it/ontology/cnr/individuo/prodotto/ID11498
Three functional subdomains of the Escherichia coli FtsQ protein are involved in its interaction with the other division proteins. (Articolo in rivista)
- Type
- Label
- Three functional subdomains of the Escherichia coli FtsQ protein are involved in its interaction with the other division proteins. (Articolo in rivista) (literal)
- Anno
- 2007-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1099/mic.0.2006/000265-0 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- D'Ulisse V; Fagioli M; Ghelardini P; Paolozzi L. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Dipartimento di Biologia Universita` 'Tor Vergata'
Laboratorio di Biologia e Patologia Molecolari del CNR di Roma, Italy (literal)
- Titolo
- Three functional subdomains of the Escherichia coli FtsQ protein are involved in its interaction with the other division proteins. (literal)
- Abstract
- FtsQ, an essential protein for the Escherichia coli divisome assembly, is able to interact with various
division proteins, namely FtsI, FtsL, FtsN, FtsB and FtsW. In this paper, the FtsQ domains involved in
these interactions were identified by two-hybrid assays and co-immunoprecipitations. Progressive
deletions of the ftsQ gene suggested that the FtsQ self-interaction and its interactions with the
other proteins are localized in three periplasmic subdomains: (i) residues 50-135 constitute one of
the sites involved in FtsQ, FtsI and FtsN interaction, and this site is also responsible for FtsW
interaction; (ii) the FtsB interaction is localized between residues 136 and 202; and (iii) the FtsL
interaction is localized at the very C-terminal extremity. In this third region, the interaction site for
FtsK and also the second site for FtsQ, FtsI, FtsN interactions are located. As far as FtsW is
concerned, this protein interacts with the fragment of the FtsQ periplasmic domain that spans
residues 67-75. In addition, two protein subdomains, one constituted by residues 1-135 and the
other from 136 to the end, are both able to complement an ftsQ null mutant. Finally, the unexpected
finding that an E. coli ftsQ null mutant can be complemented, at least transiently, by the
Streptococcus pneumoniae divIB/ftsQ gene product suggests a new strategy for investigating the
biological significance of protein-protein interactions. (literal)
- Prodotto di
- Autore CNR
Incoming links:
- Autore CNR di
- Prodotto
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi