http://www.cnr.it/ontology/cnr/individuo/prodotto/ID11397
The desaturase from Bacillus subtilis a promising tool for the selective olefination of phospholipids. (Articolo in rivista)
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- Label
- The desaturase from Bacillus subtilis a promising tool for the selective olefination of phospholipids. (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.jbiotec.2005.07.008 (literal)
- Alternative label
Bonamore A .; Macone A.; Colotti G.; Matarese R.M.; Boffi A. (2006)
The desaturase from Bacillus subtilis a promising tool for the selective olefination of phospholipids.
in Journal of biotechnology; ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, AMSTERDAM (Paesi Bassi)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Bonamore A .; Macone A.; Colotti G.; Matarese R.M.; Boffi A. (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1. Univ Roma La Sapienza, Dept Biochem Sci, I-00185 Rome, Italy
2. CNR, Inst Mol Biol & Pathol, I-00185 Rome, Italy (literal)
- Titolo
- The desaturase from Bacillus subtilis a promising tool for the selective olefination of phospholipids. (literal)
- Abstract
- The Delta 5-desaturase from Bacillus subtilis has been cloned in Escherichia coli BL21 cells and its enzyme activity has been investigated as a function of temperature and oxygenation by analyzing methyl ester adducts from the total lipid extract in GC-MS measurements. The present data bring out that the activity of recombinant Delta 5-desaturase, at 20-22 degrees C and 20% oxygen, is surprisingly high yielding 22% of C16:1,Delta 5 (5-cis-palmitoleic acid) and 13% C18:2,Delta 5 Delta 11 (efedrenic acid). Lower amounts of other mono- and doubly-Delta 5-unsaturated fatty acids were also detected. These findings demonstrate that Delta 5-desaturase can accept a multiplicity of substrates and is endowed with an unprecedented activity among other acyl-lipid desaturases thus representing a unique tool for the production of rare Delta 5 unsaturated fatty acid derivatives. (literal)
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